Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua
This study was carried out to isolate the active antibacterial compounds from marine sponge Neopetrosiaexigua. Using disc diffusion methodm methanol extract of N. exigua showed significant antibacterial activity (The inhibition zone against Staphylococcus aureus was 19.5 mm). sequesntially, liquid-l...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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2010
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| Online Access: | http://irep.iium.edu.my/12158/1/Icastcover.pdf http://irep.iium.edu.my/12158/5/isolation_of_antibacterial_compounds_from_dichloromethane_fraction_of_Malaysian_sponges_Neopetrosiaexigua.pdf |
| _version_ | 1825645301770223616 |
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| author | Qaralleh, Haitham Syed Osman Idid, Syed Zahir Idid Saad, Shahbudin Darnis, Deny Susanti |
| author_facet | Qaralleh, Haitham Syed Osman Idid, Syed Zahir Idid Saad, Shahbudin Darnis, Deny Susanti |
| author_sort | Qaralleh, Haitham |
| collection | IIUM |
| description | This study was carried out to isolate the active antibacterial compounds from marine sponge Neopetrosiaexigua. Using disc diffusion methodm methanol extract of N. exigua showed significant antibacterial activity (The inhibition zone against Staphylococcus aureus was 19.5 mm). sequesntially, liquid-liquid extraction was performed using solvenants with different polarity (n-hexane, carbon tetrachloride, dicholoromathane, n-butanol and water in order to isolate the active antibacterial compounds. Bassed on the biological guided fractionation results, dichloromathane and n-butanol fractions showed the hisgest antimicrobial activity. Purification of dichloromathane fraction was done by using SephadexLH-20, normal phase column chromatographyand reverse phase column chromatography to yeild three compounds (1-3). All compounds were tested for their antibacterial activity against Staphyloccus aureus. The results showed that 3 exhibited significant antibacterial activity. Research is still in progress to elucidate the structures of the islated compounds. |
| first_indexed | 2024-03-05T22:45:41Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:12158 |
| institution | International Islamic University Malaysia |
| language | English English |
| last_indexed | 2024-03-05T22:45:41Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:121582012-01-17T05:12:49Z http://irep.iium.edu.my/12158/ Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua Qaralleh, Haitham Syed Osman Idid, Syed Zahir Idid Saad, Shahbudin Darnis, Deny Susanti Q Science (General) This study was carried out to isolate the active antibacterial compounds from marine sponge Neopetrosiaexigua. Using disc diffusion methodm methanol extract of N. exigua showed significant antibacterial activity (The inhibition zone against Staphylococcus aureus was 19.5 mm). sequesntially, liquid-liquid extraction was performed using solvenants with different polarity (n-hexane, carbon tetrachloride, dicholoromathane, n-butanol and water in order to isolate the active antibacterial compounds. Bassed on the biological guided fractionation results, dichloromathane and n-butanol fractions showed the hisgest antimicrobial activity. Purification of dichloromathane fraction was done by using SephadexLH-20, normal phase column chromatographyand reverse phase column chromatography to yeild three compounds (1-3). All compounds were tested for their antibacterial activity against Staphyloccus aureus. The results showed that 3 exhibited significant antibacterial activity. Research is still in progress to elucidate the structures of the islated compounds. 2010-11-27 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/12158/1/Icastcover.pdf application/pdf en http://irep.iium.edu.my/12158/5/isolation_of_antibacterial_compounds_from_dichloromethane_fraction_of_Malaysian_sponges_Neopetrosiaexigua.pdf Qaralleh, Haitham and Syed Osman Idid, Syed Zahir Idid and Saad, Shahbudin and Darnis, Deny Susanti (2010) Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Malaysia. http://www.iium.edu.my/icast/2010/upload/3rd%20IIUM-iCAST%202010%20Abstract%20Book.pdf |
| spellingShingle | Q Science (General) Qaralleh, Haitham Syed Osman Idid, Syed Zahir Idid Saad, Shahbudin Darnis, Deny Susanti Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua |
| title | Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua |
| title_full | Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua |
| title_fullStr | Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua |
| title_full_unstemmed | Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua |
| title_short | Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua |
| title_sort | isolation of antibacterial compounds from dichloromethane fraction of malaysian sponges neopetrosiaexigua |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/12158/1/Icastcover.pdf http://irep.iium.edu.my/12158/5/isolation_of_antibacterial_compounds_from_dichloromethane_fraction_of_Malaysian_sponges_Neopetrosiaexigua.pdf |
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