| Summary: | Newcastle disease (ND) is one of the diseases that cause significant
economic losses due to the nature of the disease with high mortality in poultry
especially in commercial poultry. This study aims to determine the organ lesions
in broiler chickens macroscopic and microscopic infected by virulent ND virus
isolates ND-MHW/SLTG/2010. A total of 6 chickens were kept without
vaccination by the age of 14 days and then infected with 0.5 ml of virus isolates in
intratrachealis ND-MHW/SLTG/2010 when chicken was 17 days. Six number of
broiler chickens is without treatment used as a negative control. Four days after
the infection, one of the chickens that showed clinical symptoms ND were
necropsed to observe the macroscopic finding in infected chicken. Cerebrum,
trachea, proventriculus, intestinum and lung histopathology were taken and made
preparations for microscopic examination with Hematoxylin and eosin staining (H
& E). Reidentification ND virus is a virus suspension of scouring the lung that
have been infected chicken isolates ND-MHW/SLTG/2010. ND virus suspension
was then inoculated at 10 days old TAB. TAB showing the death of the embryo
after 2 days stored in the refrigerator, then collected and carried reidentification
allantois fluid using hemagglutination assay (HA) and hemagglutination inhibition
(HI) with the specific serum anti-ND. Macroscopic lesions were observed on the
hemoraghi necrotic intestine, petechial hemoraghi proventriculus and congestion
in the trachea, lung hemoraghi and meninges. Based on the histopathological
picture of the proventriculus showed necrotic foki hemoraghi. Hemoraghi the
trachea. Intestinum showed necrotic and hemoraghi Foki mucosa, villi shortened.
Found kuffing perivascular lymphocytic and vakuolisasi the cerebrum.
Hemagglutination test results show positive results that the formation of
aggregates as dashed to pieces erythrocytes indicates this virus capable
hemagglutination erythrocytes is one of the characteristics of the Newcastle virus.
The results of hemagglutination inhibition test positive with specific serum
agglutination ND formation or erythrocyte sedimentation means that the virus is
actually a virus Newcastle.
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