STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN ISOLAT ND-MHW/SLTG/2010
Newcastle disease (ND) is one of the diseases that cause significant economic losses due to the nature of the disease with high mortality in poultry especially in commercial poultry. This study aims to determine the organ lesions in broiler chickens macroscopic and microscopic infected by virulen...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1797033626670465024 |
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| author | PUSPITA, AGNISA NUR Asmara, Widya |
| author_facet | PUSPITA, AGNISA NUR Asmara, Widya |
| author_sort | PUSPITA, AGNISA NUR |
| collection | UGM |
| description | Newcastle disease (ND) is one of the diseases that cause significant
economic losses due to the nature of the disease with high mortality in poultry
especially in commercial poultry. This study aims to determine the organ lesions
in broiler chickens macroscopic and microscopic infected by virulent ND virus
isolates ND-MHW/SLTG/2010. A total of 6 chickens were kept without
vaccination by the age of 14 days and then infected with 0.5 ml of virus isolates in
intratrachealis ND-MHW/SLTG/2010 when chicken was 17 days. Six number of
broiler chickens is without treatment used as a negative control. Four days after
the infection, one of the chickens that showed clinical symptoms ND were
necropsed to observe the macroscopic finding in infected chicken. Cerebrum,
trachea, proventriculus, intestinum and lung histopathology were taken and made
preparations for microscopic examination with Hematoxylin and eosin staining (H
& E). Reidentification ND virus is a virus suspension of scouring the lung that
have been infected chicken isolates ND-MHW/SLTG/2010. ND virus suspension
was then inoculated at 10 days old TAB. TAB showing the death of the embryo
after 2 days stored in the refrigerator, then collected and carried reidentification
allantois fluid using hemagglutination assay (HA) and hemagglutination inhibition
(HI) with the specific serum anti-ND. Macroscopic lesions were observed on the
hemoraghi necrotic intestine, petechial hemoraghi proventriculus and congestion
in the trachea, lung hemoraghi and meninges. Based on the histopathological
picture of the proventriculus showed necrotic foki hemoraghi. Hemoraghi the
trachea. Intestinum showed necrotic and hemoraghi Foki mucosa, villi shortened.
Found kuffing perivascular lymphocytic and vakuolisasi the cerebrum.
Hemagglutination test results show positive results that the formation of
aggregates as dashed to pieces erythrocytes indicates this virus capable
hemagglutination erythrocytes is one of the characteristics of the Newcastle virus.
The results of hemagglutination inhibition test positive with specific serum
agglutination ND formation or erythrocyte sedimentation means that the virus is
actually a virus Newcastle. |
| first_indexed | 2024-03-13T22:57:04Z |
| format | Thesis |
| id | oai:generic.eprints.org:121737 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:57:04Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1217372018-05-25T06:50:22Z https://repository.ugm.ac.id/121737/ STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN ISOLAT ND-MHW/SLTG/2010 PUSPITA, AGNISA NUR Asmara, Widya Veterinary Virology Newcastle disease (ND) is one of the diseases that cause significant economic losses due to the nature of the disease with high mortality in poultry especially in commercial poultry. This study aims to determine the organ lesions in broiler chickens macroscopic and microscopic infected by virulent ND virus isolates ND-MHW/SLTG/2010. A total of 6 chickens were kept without vaccination by the age of 14 days and then infected with 0.5 ml of virus isolates in intratrachealis ND-MHW/SLTG/2010 when chicken was 17 days. Six number of broiler chickens is without treatment used as a negative control. Four days after the infection, one of the chickens that showed clinical symptoms ND were necropsed to observe the macroscopic finding in infected chicken. Cerebrum, trachea, proventriculus, intestinum and lung histopathology were taken and made preparations for microscopic examination with Hematoxylin and eosin staining (H & E). Reidentification ND virus is a virus suspension of scouring the lung that have been infected chicken isolates ND-MHW/SLTG/2010. ND virus suspension was then inoculated at 10 days old TAB. TAB showing the death of the embryo after 2 days stored in the refrigerator, then collected and carried reidentification allantois fluid using hemagglutination assay (HA) and hemagglutination inhibition (HI) with the specific serum anti-ND. Macroscopic lesions were observed on the hemoraghi necrotic intestine, petechial hemoraghi proventriculus and congestion in the trachea, lung hemoraghi and meninges. Based on the histopathological picture of the proventriculus showed necrotic foki hemoraghi. Hemoraghi the trachea. Intestinum showed necrotic and hemoraghi Foki mucosa, villi shortened. Found kuffing perivascular lymphocytic and vakuolisasi the cerebrum. Hemagglutination test results show positive results that the formation of aggregates as dashed to pieces erythrocytes indicates this virus capable hemagglutination erythrocytes is one of the characteristics of the Newcastle virus. The results of hemagglutination inhibition test positive with specific serum agglutination ND formation or erythrocyte sedimentation means that the virus is actually a virus Newcastle. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed PUSPITA, AGNISA NUR and Asmara, Widya (2013) STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN ISOLAT ND-MHW/SLTG/2010. Masters thesis, Universitas Gadjah Mada. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61836 |
| spellingShingle | Veterinary Virology PUSPITA, AGNISA NUR Asmara, Widya STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN ISOLAT ND-MHW/SLTG/2010 |
| title | STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM
BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN
ISOLAT ND-MHW/SLTG/2010 |
| title_full | STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM
BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN
ISOLAT ND-MHW/SLTG/2010 |
| title_fullStr | STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM
BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN
ISOLAT ND-MHW/SLTG/2010 |
| title_full_unstemmed | STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM
BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN
ISOLAT ND-MHW/SLTG/2010 |
| title_short | STUDI LESI MAKROSKOPIS DAN MIKROKOPIS PADA AYAM
BROILER YANG DIINFEKSI VIRUS NEWCASTLE DISEASE VIRULEN
ISOLAT ND-MHW/SLTG/2010 |
| title_sort | studi lesi makroskopis dan mikrokopis pada ayam broiler yang diinfeksi virus newcastle disease virulen isolat nd mhw sltg 2010 |
| topic | Veterinary Virology |
| work_keys_str_mv | AT puspitaagnisanur studilesimakroskopisdanmikrokopispadaayambroileryangdiinfeksivirusnewcastlediseasevirulenisolatndmhwsltg2010 AT asmarawidya studilesimakroskopisdanmikrokopispadaayambroileryangdiinfeksivirusnewcastlediseasevirulenisolatndmhwsltg2010 |