| Summary: | The antibacterial power is tested by infusing 50 µl Apis mellifera honey at
20%, 30%, 40%, and 50% concentration,
Periodontal disease is a disease of periodontal tissue which caused by
subgingival plaque bacteria. Aggregatibacter actinomycetemcomitans is a
facultative anaerobic gram-negative bacteria that leads to periodontal disease.
Apis mellifera honey, derived from Apis mellifera honey bee, is an antibacterial
agent which is most commonly found in Indonesia. The aim of this study is to
determine the effect of Apis mellifera honey on the growth of Aggregatibacter
actinomycetemcomitans bacteria.
sterile distilled water as negative
control, and 0,2% chlorhexidine as positive control into the well hole of 5 petri
dishes (n=5) MHA media planted with Aggregatibacter actinomycetemcomitans.
The MHA media were then incubated for 24 hours at 37oC temperature. The
inhibition zones formed were measured using caliper.
The mean and standard deviation results of the inhibition zones diameter of
Apis mellifera honey at 20%, 30%, 40%, and 50% concentration are 2.51 ± 0.439,
3.98 ± 0.599, 5.25 ± 0.220, and 6.55 ± 0.990. Kruskal-Wallis test showed that
Apis mellifera honey at 20%, 30%, 40%, and 50% concentration could inhibit the
Aggregatibacter actinomycetemcomitans growth. Mann-Whitney test showed
there were significant differences among the concentrations.
The conclusion of this study is Apis mellifera honey at 20%, 30%, 40%, and
50% concentration are able to inhibit the growth of Aggregatibacter
actinomycetemcomitans bacteria.
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