KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus bancanus DAN Tarsius dianae
Tarsius sp. is a small nocturnal primate that found in Indonesia, which decreased in population, and needs conservation attempt to preserve them. Species of tarsius is very difficult to identify because of their morphology are nearly the same and for its success of conservation, it needs encoding ge...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: |
| _version_ | 1797033658573389824 |
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| author | , RUDIAR ANISA , Dr. drh. Rini Widayanti, MP. |
| author_facet | , RUDIAR ANISA , Dr. drh. Rini Widayanti, MP. |
| author_sort | , RUDIAR ANISA |
| collection | UGM |
| description | Tarsius sp. is a small nocturnal primate that found in Indonesia, which
decreased in population, and needs conservation attempt to preserve them. Species
of tarsius is very difficult to identify because of their morphology are nearly the
same and for its success of conservation, it needs encoding gene for them. The
main objective of this research is to study about the genetic diversity of NADH
Dehydrogenase Sub-unit 6 (ND6) encoding gene on Tarsius sp. DNA samples are
isolated from the thigh muscle of three Tarsius bancanus bancanus from
Lampung and a Tarsius dianae from Center of Celebes. DNA Samples than
amplificated with Polymerase Chain Reaction (PCR) technique used ND6F and
ND6R primer for T. b. bancanus samples and ND6F lama and ND6R primer for
T. dianae samples. Amplification process produces PCR product with 586 base
pair (bp) (T. b. bancanus) and 662 bp (T. dianae) which than sequenced.
Sequencing result then analyzed using MEGA 5.05 version program and
compared with some other Tarsius sp. species obtained from Genbank. Analysis
result showed that the genetic distance based on nucleotide sequence was 28,7%
and based on amino acid sequence was 27,1%. Construction of phylogeny tree
based on nucleotide and amino acid of ND6 gene with Neighbor-Joining method
could differentiate Tarsius bancanus bancanus and Tarsius dianae with Tarsius
sp. as comparing species. |
| first_indexed | 2024-03-13T22:57:49Z |
| format | Thesis |
| id | oai:generic.eprints.org:121897 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:57:49Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1218972016-03-04T08:42:38Z https://repository.ugm.ac.id/121897/ KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus bancanus DAN Tarsius dianae , RUDIAR ANISA , Dr. drh. Rini Widayanti, MP. ETD Tarsius sp. is a small nocturnal primate that found in Indonesia, which decreased in population, and needs conservation attempt to preserve them. Species of tarsius is very difficult to identify because of their morphology are nearly the same and for its success of conservation, it needs encoding gene for them. The main objective of this research is to study about the genetic diversity of NADH Dehydrogenase Sub-unit 6 (ND6) encoding gene on Tarsius sp. DNA samples are isolated from the thigh muscle of three Tarsius bancanus bancanus from Lampung and a Tarsius dianae from Center of Celebes. DNA Samples than amplificated with Polymerase Chain Reaction (PCR) technique used ND6F and ND6R primer for T. b. bancanus samples and ND6F lama and ND6R primer for T. dianae samples. Amplification process produces PCR product with 586 base pair (bp) (T. b. bancanus) and 662 bp (T. dianae) which than sequenced. Sequencing result then analyzed using MEGA 5.05 version program and compared with some other Tarsius sp. species obtained from Genbank. Analysis result showed that the genetic distance based on nucleotide sequence was 28,7% and based on amino acid sequence was 27,1%. Construction of phylogeny tree based on nucleotide and amino acid of ND6 gene with Neighbor-Joining method could differentiate Tarsius bancanus bancanus and Tarsius dianae with Tarsius sp. as comparing species. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , RUDIAR ANISA and , Dr. drh. Rini Widayanti, MP. (2013) KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus bancanus DAN Tarsius dianae. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61996 |
| spellingShingle | ETD , RUDIAR ANISA , Dr. drh. Rini Widayanti, MP. KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus bancanus DAN Tarsius dianae |
| title | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA
Tarsius bancanus bancanus DAN Tarsius dianae |
| title_full | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA
Tarsius bancanus bancanus DAN Tarsius dianae |
| title_fullStr | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA
Tarsius bancanus bancanus DAN Tarsius dianae |
| title_full_unstemmed | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA
Tarsius bancanus bancanus DAN Tarsius dianae |
| title_short | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA
Tarsius bancanus bancanus DAN Tarsius dianae |
| title_sort | kajian keragaman genetik gen penyandi nadh dehydrogenase sub unit 6 nd6 pada tarsius bancanus bancanus dan tarsius dianae |
| topic | ETD |
| work_keys_str_mv | AT rudiaranisa kajiankeragamangenetikgenpenyandinadhdehydrogenasesubunit6nd6padatarsiusbancanusbancanusdantarsiusdianae AT drdrhriniwidayantimp kajiankeragamangenetikgenpenyandinadhdehydrogenasesubunit6nd6padatarsiusbancanusbancanusdantarsiusdianae |