| Summary: | Psidium guajava is a plant that grows in Indonesia. Psidium guajava�s
leaf is suspected that have got efficacy as an antibacterial agent. The aim of this
study is to determine the effect of concentration of extract of Psidium guajava�s
leaf in the ultrasonic scaler coolant water to inhibit growth of Porhyromonas
gingivalis.
Twenty-one tubes of bacterial suspension were sprayed with an extract of
Psidium guajava�s leaf which concentration i.e. 0.009%, 0.019%, 0.039%,
0.078%, 0.156%, distilled water as a negative control and chlorhexidine
gluconate 0.0034% as a positive control through ultrasonic scaler. The 0.2 ml of
suspension bacterial and extract liquid was taken and was trim on the blood agar
plate with a glass spreader. Then, blood agar plates were incubated for 48 hours,
with temperature of 37�C in the anaerobic incubator. Colonies formed were
counted by 3 observers and continued with statistical analysis.
The results of one way Anova test showed that concentration of Psidium
guajava�s leaf in the ultrasonic scaler coolant water has significant effect on the
inhibition of bacterial Porphyromonas gingivalis growth (p<0.05). The results of
LSD test of showed that there were significant differences between all treatment
groups against distilled water and chlorhexidine gluconate 0.0034% (p<0.05),
except in the concentration of 0.156%, there was not significant difference against
chlorhexidine gluconate 0.0034% (p>0.05). The conclusion of this study is
concentration of extract of Psidium guajava�s leaf in the ultrasonic scaler coolant
water has got an effect on the inhibition of bacterial Porhyromonas gingivalis
growth.
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