VALIDASI METODE ANALISIS DELTAMETHRIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) : Penggunaan Kolom C18, dengan Fase Gerak Asetonitril 80% dalam Akuabides, Laju Alir Fase Gerak 1,6ml/menit, dan Panjang Gelombang Detektor UV-Vis 190 nm

Deltamethrin was a common pesticide used in plants and animals. Research about this pesticide has been done with various methods. The purpose of this research was to validate the alternative test method of deltamethrin using High Performance Liquid Chromatography (HPLC). This research used pure delt...

Full description

Bibliographic Details
Main Authors: , LEOISNA NIRBHITA FAJRIN, , drh. R. Gagak Donny Satria, M.P., M.Pd.
Format: Thesis
Published: [Yogyakarta] : Universitas Gadjah Mada 2013
Subjects:
ETD
Description
Summary:Deltamethrin was a common pesticide used in plants and animals. Research about this pesticide has been done with various methods. The purpose of this research was to validate the alternative test method of deltamethrin using High Performance Liquid Chromatography (HPLC). This research used pure deltamethrin. The tool in this research was HPLC Shimazu 6.1 with 80% acetonitrile in aquabides for mobile phase, flow rate 1.6 ml / min and detector wavelength 190 nm. The data were analyzed by calculated the average, standard deviation, and relative standard deviation. The results showed the average area for each concentration of 0.1 ug/ml, 0.25 ug/ml, 0.5 ug/ml, 1 ug/ml, 2 μg/ml, and 5 ug/ml, were 52806.33, 119070.3, 220292, 449448.7, 856049.3, and 2,177,558, with a linear equation y = 433070x + 7092, R = 0,999. It can be concluded that the method is valid based on specificity, limit of detection, limit of quantification, precision, acuration, and linearity.