Summary: | The purpose of this study is to observe the diversity and the population density of
rhizobacteria in F. oxysporum f.sp. cubense uninfected soil and infected soil, to get and examine rhizobacteria and endophytic bacteria which can suppress F. oxysporum f.sp. cubense tropical race 4 with can suppress fusarium wilt disease on banana, and for identification the antagonistic bacterial isolates and compare with the bacteria sequences to NCBI GeneBank database. The research was done in Laboratory of Clinical Plant Disease and Plant Bacteriology, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta. Six soil samples (4 uninfected soil samples, 2 infected soil samples) and 2 bacterial isolates collection Laboratory of Clinical Plant Disease
was used. The result is that the diversity and population density of rhizobacterial in F.
oxysporum f.sp. cubense uninfected soil is higher than infected soil. There was found out 3 rhizobacterial isolates (CJ20GGP, CJ20NTF, SRP/I) and 2 endophytic isolates (6P2, 2P5) can suppress F. oxysporum f.sp. cubense tropical race 4 that can suppress fusarium wilt disease on banana. Isolates of 6P2, CJ20GGP, SRP/I and CJ20NTF was close related to Bacillus amyloliquefaciens, while the isolate of 2P5 was close related to Bacillus stratosphericus.
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