KAJIAN MOLEKULER AYAM KEDU, BURAS DAN CEMANI MENGGUNAKAN POLYMERASE CHAIN REACTION - RANDOM AMPLIFIED POLYMORPHIC DEOXYRIBONUCLEIC ACID (PCR-RAPD)

Kedu chicken, domestic poultry, and cemani is necessary germplasm preserved Indonesia. Information about the genetic diversity of the chicken is still limited and research is still needed to be. One attempt to assess the diversity of the chicken genetic information, the research can be done by the m...

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Bibliographic Details
Main Authors: , WINDA AYUNINGTYAS, , Dr. drh. Trini Susmiati, M.P.
Format: Thesis
Published: [Yogyakarta] : Universitas Gadjah Mada 2013
Subjects:
ETD
Description
Summary:Kedu chicken, domestic poultry, and cemani is necessary germplasm preserved Indonesia. Information about the genetic diversity of the chicken is still limited and research is still needed to be. One attempt to assess the diversity of the chicken genetic information, the research can be done by the method of Polymerase Chain Reaction-Random Amplified Polymorphic Deoxyribonucleic Acid (PCR-RAPD) using primers 3 (5� GTA GAC CCG T 3�) and 5 (5' AAC GCG CAA C 3'). The purpose of this study was to examine the potential genetic diversity of kedu chicken, domestic poultry, and cemani as seen from the specific DNA fragments by the method of Polymerase Chain Reaction-Random Amplified Polymorphic Deoxyribonucleic Acid (PCR-RAPD), and to assess whether there is kinship between the kedu chicken and cemani obtained from Temanggung, and domestic poultry obtained from Purworejo, Central Java. The results showed the existence of genetic diversity within populations and among populations, but can not be used as genetic markers. The closest genetic distance in this study are red kedu to white kedu. Farthest genetic distance is between domestic poultry to cemani and cemani to red kedu.