| Summary: | Staphylococcus aureus is a major bacterial pathogen causing subclinical
mastitis on dairy cow. Subclinical mastitis is usually treated by using antibiotics.
Improper use of antibiotics causes bacterial resistance to certain antibiotics. This
study aimed to determine the resistance of S. aureus isolated from milk of dairy
cows against methicillin, erythromycin, doxcycycline , gentamycin, ciprofloxacin
and penicillin..
17 Staphylococcus aureus isolated from milk of dairy cows were used in
this study. Identification of S. aureus based on the growths of bacteria the Todd-
Hewit Broth (THB), mannitol salt agar (MSA), blood agar plate (PAD), Gram
staining, catalase and coagulase test. Sensitivity test against antibiotics were done
by using the media of Müller Hinton agar (MHA) by Disc Diffusion of Kirby-
Bauer test. The kinds of antibiotics used in the sensitivity tests were methicillin 5
μg, erythromycin 15 μg , doxycycline 30 μg, gentamycin 10 μg, ciprofloxacin 5
μg and penicillin G 10 μg. The measurements of inhibition zones on the Müller
Hinton against various antibiotics were compared to the standard Kirby-Bauer
table.
The results showed that all Staphylococcus aureus isolates were resistance
to methicillin (5,88%), penicillin G (35,30%), erythromycin (0%), doxycycline
(0%), gentamycin (5,88%) and ciprofloxacin (0%). From the research result can
be concluded that subclinical mastitis caused by S. aureus in dairy cow in
Baturraden can be treated use erythromycin, doxycycline and ciprofloxacin.
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