| Summary: | Study on diversity among eight Dendrobium spp. orchids is necessary for orchid
breeders in order to plant crossing efficiency. Highly similarity among Dendrobium spp.
would increase crossing opportunity. The objectives of this study were to grouping
orchid genotypes into clusters according to their similarity as measured by DNA
banding patterns. Genetic similarity among 10 orchid genotypes were analysed based on
85 bands generated from five RAPD primers. The result showed that genetic diversity
within species indicated by percentage of polymorphic loci was in range of 11.76 to
40%. Cluster analysis based on molecular markers showed that the Dendrobiums were
divided into six groups at a distance of 7.5 in dendogram, where is first group consisted
of D. spectabile and D. macrophyllum, second group consisted of D. lasianthera, third
group consisted of D. antennatum, D. discolor, and D. stratiotes, fourth group consisted
of D. smillieae and D. crumenatum, fifth group consisted of D. signatum, and sixth
group consisted of D. fimbriatum. Two Dendrobium spesies from another country (D.
fimbriatum and D. signatum) made a group outside of Dendrobium of Indonesia�s
group, indicating that both spesies have distant genetic similarity with Dendrobium
spesies of Indonesia. Result of grouping based on molecular markers is different from
grouping based on morphological observation, probably due to environment effects.
|
|---|