| Summary: | Ettawa cross breed goats is an alternative to dairy milk. Goat milk also
contains vitamins and minerals are higher than cow's milk and can be taken for a
person who are intolerant to lactose. The main problem on the production of goat
milk is mastitis. Bacillus sp is one of Gram-positive rod bacterium which causing
mastitis are still rarely studied. This study aimed to isolate and identify the
bacteria Gram positive rods especially Bacillus sp from subclinical goat�s milk.
A total of 40 samples from 20 ettawa cross breed goats were taken from
the farm "Sahabat Alam Sejati" Kalasan, Sleman, Yogyakarta tested with IPB-1
reagent to decide mastitis status. Milk sample with positive subclinical mastitis
result then continued with isolated on Blood Agar Plate media (PAD) and
incubated for 18-24 hours at 37oC. Observed colonies were then grouped based on
colony morphology and Gram staining performed. From a total of 125 colonies at
PAD, 47 isolates of Gram-positive rods continued motility test, the growth of
aerobic and anaerobic, and spore staining.
In this research has found 4 species rods Gram positive bacteria, namely
Bacillus cereus were 9 isolates (19.2%), Bacillus subtilis 1 isolates (2.1%),
Bacillus pantothenticus as 6 isolates (12.8) and Bacillus sp of 31 isolates (65.9%).
Bacillus sp is the most Gram-positive rods species was found in this research.
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