| Summary: | Oral wound is one of the most prevalent inflammatory diseases in the
society with around 15 � 20% of the world population affected. Gamavuton-0
(GVT-0) is an analogue compound of curcumin which possess a variety of
pharmacological effects such as antioxidant, anti-microbial and antiinflammatory.
Effect of GVT-0 on wound healing process has yet been observed.
The aim of this study was to develop and create the optimum formula of GVT-0
buccoadhesive film using chitosan and PVA as well as to investigate its effect
towards the number of polymorphonuclear (PMN) cells on Wistar rats.
Film formulas were determined by using simplex lattice design method
with software Design Expert 7.1.3. which resulted into 8 runs. The prepared films
were characterized by using its film weight, thickness, surface pH, swelling index,
folding endurance, drug content, and in vitro drug release profile. These
characterizations were used to obtain the optimum combination formula of
chitosan and PVA which then were analyzed to study buccal mucosa permeation
transport and ex vivo residence time. In vivo study was conducted involving 48
male 2 � 3 months old and 200 � 300 grams in weight Wistar rats which then
were divided randomly into control groups and treated group. The rats in all
groups were sacrificed on day 1, 3, 5 and 7 after injury, then the wounded tissue
were processed into histology specimen with HE staining.
The optimum combination result of 1.518% chitosan and 1.982 % PVA
showed that all films produced were good in appearance and flexibility. The
weight and thickness were found to be uniform with the drug content ranging from
93 � 96 %. Swelling index showed favorable result with its pH ranging from 5 � 6.
In vitro drug release of the optimum formulation showed that about 48.823 ±
0.348% of GVT-0 was released with a residence time of 153 ± 2.870 minutes.
Buccal permeation test through rabbit buccal mucosa was found to be 2.020% ±
0.067 %. This study concluded that, the application of GVT-0 buccoadhesive films
significantly (p<0.050) showed the ability to decrease the number of PMN cells
significantly during wound healing process on buccal mucosa.
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