| Summary: | aims The of this study was to identify and characterize potential probiotics
with the ability to synthesize bioactive compounds CLA. Five isolates of LAB
isolated from faeces of infants who consumed breast milk as the only source of
diet were grown on L-cysteine-supplemented MRS Agar and incubated on 37°C
for 48 hours. Colonies grew on this media were then phenotypically identified
based on morphological and biochemichal, followed by genotypic identification.
Phenotypic identifications based on Gram staining, catalase, motility, spore
forming, CO2 production, and the ability to grow on different temperatures, pH
and concentration of salt. Genotypic identification relied on the amplification of
sequence of 16S rRNA gene. The potential application of selected isolates for
probiotics was evaluated based on the ability to grow on media with low pH and
bile salts, the ability to inhibit the growth of pathogen, in vitro adherence ability
on pig gastric mucin and to utilize inulin as the only carbon source. Based on the
identification of cellular, biochemical and 16S rRNA gene analysis, isolates AP
and AG were classified as Lactobacillus casei (99%), whereas isolates AA, BE
and BK were classified as Pediococcus acidilactici (99%). In vitro evaluation of
five isolates showed potential candidate as probiotic through several test
parameters. Evaluation on the ability to use prebiotic inulin as carbon source
showed that Lactobacillus casei (strain AP and AG) and Pediococcus acidilactici
strains BE had the ability to degrade inulin as a prebiotic. Selection of candidate
probiotic isolates to synthesize Conjugated Linoleic Acid (CLA) from free
linoleic acid showed that Lactobacillus casei strain AP was able to convert more
than 60% of free linoleic acid to CLA in the media. The data obtained indicated
that the selected isolate Lactobacillus casei strain AP is potential candidate for
probiotic application and produce CLA.
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