VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI
Energy drinks containing caffeine, benzoate, aspartame, acesulfame, and cyclamate widely circulated and consumed by public, but there were no analytical methods are to determine the levels of caffeine, benzoate, aspartame, acesulfame, and cyclamate simultaneously using HPLC with UV-PDA detector. The...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| author | , Dwi Setio Purnomo , Prof. Dr. Sudibyo Martono, M.S., Apt. |
| author_facet | , Dwi Setio Purnomo , Prof. Dr. Sudibyo Martono, M.S., Apt. |
| author_sort | , Dwi Setio Purnomo |
| collection | UGM |
| description | Energy drinks containing caffeine, benzoate, aspartame, acesulfame, and
cyclamate widely circulated and consumed by public, but there were no analytical
methods are to determine the levels of caffeine, benzoate, aspartame, acesulfame,
and cyclamate simultaneously using HPLC with UV-PDA detector. The aim of
this study was to obtain HPLC-UV-PDA method which met the validation
parameters namely selectivity, linearity, accuracy, precision, limit of detection as
well as limit of quantitation, and robustness.
Potassium acesulfame, sodium cyclamate, caffeine, sodium benzoate, and
aspartame were detected at a wavelength of 225, 200, 272, 225, and 200 nm,
respectively. The HPLC system consisted of Kromasil C18 (250 x 4,6 mm i.d.,
10 μm particle size) at 40oC as a stationary phase, the mobile phase a mixtures of
25 mM phosphate buffer pH 4.3-methanol (80 : 20 v/v), with a flow rate of
1.5 mL/min.
The method validation showed a good selectivity with resolution > 2,0 for
all analytes. Linearity range were 4 - 24 mg/L for potassium acesulfame, caffeine,
sodium benzoate, and aspartame, while 30 - 180 mg/L for sodium cyclamate, with
correlation coefficien (r) > 0.999 for all analytes. Detection limit of potassium
acesulfame, sodium cyclamate, caffeine, sodium benzoate, aspartame were 0.21 |
| first_indexed | 2024-03-13T23:02:21Z |
| format | Thesis |
| id | oai:generic.eprints.org:123134 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:02:21Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1231342016-03-04T08:32:49Z https://repository.ugm.ac.id/123134/ VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI , Dwi Setio Purnomo , Prof. Dr. Sudibyo Martono, M.S., Apt. ETD Energy drinks containing caffeine, benzoate, aspartame, acesulfame, and cyclamate widely circulated and consumed by public, but there were no analytical methods are to determine the levels of caffeine, benzoate, aspartame, acesulfame, and cyclamate simultaneously using HPLC with UV-PDA detector. The aim of this study was to obtain HPLC-UV-PDA method which met the validation parameters namely selectivity, linearity, accuracy, precision, limit of detection as well as limit of quantitation, and robustness. Potassium acesulfame, sodium cyclamate, caffeine, sodium benzoate, and aspartame were detected at a wavelength of 225, 200, 272, 225, and 200 nm, respectively. The HPLC system consisted of Kromasil C18 (250 x 4,6 mm i.d., 10 μm particle size) at 40oC as a stationary phase, the mobile phase a mixtures of 25 mM phosphate buffer pH 4.3-methanol (80 : 20 v/v), with a flow rate of 1.5 mL/min. The method validation showed a good selectivity with resolution > 2,0 for all analytes. Linearity range were 4 - 24 mg/L for potassium acesulfame, caffeine, sodium benzoate, and aspartame, while 30 - 180 mg/L for sodium cyclamate, with correlation coefficien (r) > 0.999 for all analytes. Detection limit of potassium acesulfame, sodium cyclamate, caffeine, sodium benzoate, aspartame were 0.21 [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Dwi Setio Purnomo and , Prof. Dr. Sudibyo Martono, M.S., Apt. (2013) VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63245 |
| spellingShingle | ETD , Dwi Setio Purnomo , Prof. Dr. Sudibyo Martono, M.S., Apt. VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI |
| title | VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM
BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI |
| title_full | VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM
BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI |
| title_fullStr | VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM
BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI |
| title_full_unstemmed | VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM
BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI |
| title_short | VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR NATRIUM
BENZOAT, ASPARTAM, KALIUM ASESULFAM, NATRIUM SIKLAMAT, DAN KAFEIN DALAM MINUMAN ENERGI |
| title_sort | validasi metode kromatografi cair kinerja tinggi untuk penetapan kadar natrium benzoat aspartam kalium asesulfam natrium siklamat dan kafein dalam minuman energi |
| topic | ETD |
| work_keys_str_mv | AT dwisetiopurnomo validasimetodekromatograficairkinerjatinggiuntukpenetapankadarnatriumbenzoataspartamkaliumasesulfamnatriumsiklamatdankafeindalamminumanenergi AT profdrsudibyomartonomsapt validasimetodekromatograficairkinerjatinggiuntukpenetapankadarnatriumbenzoataspartamkaliumasesulfamnatriumsiklamatdankafeindalamminumanenergi |