| Summary: | Background: Sepsis was the most common cause of death in intensive care unit (ICU) around the world. 10% of sepsis patients had absolute thiamine deficiency upon presentation, and increased to 20% after 72 hours. Without thiamine, pyruvate can�t come into citric acid cycle in aerobic metabolism. Thiamine was expected to support metabolism process, prevent tissue and organ dysfunction.
Objective: to explore the effect of thiamine to lactic acid level in sepsis rats.
Method: 18 Sprague Dawley rats was divided into 3 groups: group K(sham+placebo), P1(CLP+placebo), and P2(CLP+Thiamine). This study conducted in 7 days and used 25 mg/kgBB thiamine dosage. Blood culture test, leucocyte level and temperature was measured for sepsis diagnostic confirmation. Temperature was measured every day during the study, blood culture test at first day post CLP and lactic acid level at day 0,1,3,7 post CLP. The data was analyzed with statistical test One Way ANOVA and paired t-test.
Result: Lactic acid level increased in all groups at the first day post CLP. The decreased level occurred at the third day in group K and P2 while lactic acid level still increased in group P1. The decreasing level was not reaching the baseline of lactic acid level.
Conclusion: Thiamine 25 mg/kgBB was not giving significant decrease of lactic acid level in sepsis rats.
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