ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL
Yolk sac infection is the primary cause of chicks mortality in the first week of life. The disease is often accompanied by inflammation of the navel (omphalitis), and the chicks appear weak, drowsy, and anorexic. Egg contaminated by bacteria and unhealed navel may be the important route of transmiss...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1797033958493388800 |
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| author | , Hana Setyawati , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D. |
| author_facet | , Hana Setyawati , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D. |
| author_sort | , Hana Setyawati |
| collection | UGM |
| description | Yolk sac infection is the primary cause of chicks mortality in the first
week of life. The disease is often accompanied by inflammation of the navel
(omphalitis), and the chicks appear weak, drowsy, and anorexic. Egg
contaminated by bacteria and unhealed navel may be the important route of
transmission of the disease. Antibiotics are often used to treat yolk sac infection.
However, the emergence of antibiotics resistant bacteria lead to more complicated
disease healing process.
The purposes of this study are to isolate and to identify yolk sac infectioncausing
bacteria in commercial broiler chicks, and to evaluate the antibiotics
susceptibility of these bacteria against commonly used antibiotics in the field to
observe the most effective therapy for the treatment of yolk sac infection. Twentytwo,
one to three days old broiler chicks with yolk sac infection were necropsied,
and bacterial isolation was made from the yolk sac. Sample of yolk sac was
inoculated in blood agar, followed by Gram stain and biochemical tests for
identification of the isolated bacteria. Antibiotics susceptibility test was conducted
based on Kirby-Bauer disc diffusion method to several antibiotics, including
amoxcycillin-clavulanic acid, enrofloxacin, tetracycline, erythromycin, and
colistin sulfate.
Gram positive and Gram negative bacteri isolated were as much as 62%
(21/34) and 38% (13/34), respectively. The most frequently Gram positive
bacteria isolated was Enterococcus spp. (44.1% (15/34)), whereas the most
frequently Gram negative bacteria isolated was Escherichia coli (11.8% (4/34)).
Bacteria isolated from this study were Enterococcus spp. (44.1%), S. aureus
(8.8%), Staphylococcus spp. (8.8%), E. coli (11.8%), Klebsiella sp. (8.8%),
Serratia sp. (8.8%), and Gram negative non Enterobacteriaceae (8.8%). The
majority of bacteria isolated were sensitive to amoxicillin-clavulanic acid
(82.3%), tetracycline (41.2%), enrofloxacin (38.2%), and were resistant to colistin
sulfate (100%), erythromycin (82.3%), tetracycline (55.9%), and enrofloxacin
(38.2%). Based on the results of this experiment, it can be concluded that there
were some sensitive and resistant bacteria isolated from infected yolk sac.
Therefore, administration of antibiotic to commercial broiler should be given
rationally in order to minimize the emergence of resistant bacteria which could
prolong the disease healing process. |
| first_indexed | 2024-03-13T23:03:14Z |
| format | Thesis |
| id | oai:generic.eprints.org:123383 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:03:14Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1233832016-03-04T08:38:39Z https://repository.ugm.ac.id/123383/ ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL , Hana Setyawati , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D. ETD Yolk sac infection is the primary cause of chicks mortality in the first week of life. The disease is often accompanied by inflammation of the navel (omphalitis), and the chicks appear weak, drowsy, and anorexic. Egg contaminated by bacteria and unhealed navel may be the important route of transmission of the disease. Antibiotics are often used to treat yolk sac infection. However, the emergence of antibiotics resistant bacteria lead to more complicated disease healing process. The purposes of this study are to isolate and to identify yolk sac infectioncausing bacteria in commercial broiler chicks, and to evaluate the antibiotics susceptibility of these bacteria against commonly used antibiotics in the field to observe the most effective therapy for the treatment of yolk sac infection. Twentytwo, one to three days old broiler chicks with yolk sac infection were necropsied, and bacterial isolation was made from the yolk sac. Sample of yolk sac was inoculated in blood agar, followed by Gram stain and biochemical tests for identification of the isolated bacteria. Antibiotics susceptibility test was conducted based on Kirby-Bauer disc diffusion method to several antibiotics, including amoxcycillin-clavulanic acid, enrofloxacin, tetracycline, erythromycin, and colistin sulfate. Gram positive and Gram negative bacteri isolated were as much as 62% (21/34) and 38% (13/34), respectively. The most frequently Gram positive bacteria isolated was Enterococcus spp. (44.1% (15/34)), whereas the most frequently Gram negative bacteria isolated was Escherichia coli (11.8% (4/34)). Bacteria isolated from this study were Enterococcus spp. (44.1%), S. aureus (8.8%), Staphylococcus spp. (8.8%), E. coli (11.8%), Klebsiella sp. (8.8%), Serratia sp. (8.8%), and Gram negative non Enterobacteriaceae (8.8%). The majority of bacteria isolated were sensitive to amoxicillin-clavulanic acid (82.3%), tetracycline (41.2%), enrofloxacin (38.2%), and were resistant to colistin sulfate (100%), erythromycin (82.3%), tetracycline (55.9%), and enrofloxacin (38.2%). Based on the results of this experiment, it can be concluded that there were some sensitive and resistant bacteria isolated from infected yolk sac. Therefore, administration of antibiotic to commercial broiler should be given rationally in order to minimize the emergence of resistant bacteria which could prolong the disease healing process. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Hana Setyawati and , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D. (2013) ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63494 |
| spellingShingle | ETD , Hana Setyawati , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D. ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL |
| title | ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB
INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL |
| title_full | ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB
INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL |
| title_fullStr | ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB
INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL |
| title_full_unstemmed | ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB
INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL |
| title_short | ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB
INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL |
| title_sort | isolasi identifikasi dan uji sensitivitas antibiotik terhadap bakteri penyebab infeksi yolk sac pada anak ayam pedaging komersial |
| topic | ETD |
| work_keys_str_mv | AT hanasetyawati isolasiidentifikasidanujisensitivitasantibiotikterhadapbakteripenyebabinfeksiyolksacpadaanakayampedagingkomersial AT profdrhcharlesranggatabbumscphd isolasiidentifikasidanujisensitivitasantibiotikterhadapbakteripenyebabinfeksiyolksacpadaanakayampedagingkomersial |