| Summary: | Alpha terpineol is a naturally alcoholic-monoterpene compound. This
component is a part of essential oil extract in plants, such as Pines (Pinus merkusii).
Alpha terpineol is a potential natural product with anticancer activity which is
proven can decrease cells growth and induces cells death. Chemotherapeutic
combination is a combined of a chemoprevention compound with chemotherapeutic
agent to increase therapeutic effect and reducing toxicity. The purpose of this study
is to determine the effect combination alpha terpineol with doxorubicin in breast
cancer cell line MCF-7
The cytotoxic assay of alpha terpineol and doxorubicin were carried out by
MTT method using alpha terpineol and doxorubicin concentration of 1,5625-50
µg/ml to determine IC50. The cytotoxic assay of their combination were carried out
by MTT method using concentration were 1/2, 1/8 and 1/20 IC50 values. Apoptotic
assay of their best combination concentration were conducted using flow
cytometryAnnexin V-Pi.
Alpha terpineol and doxorubicin showed cytotoxic effect on MCF-7 breast
cancer cell with IC50 30 µg/ml and 2 µg/ml, respectively. Based on CI values, all
combination concentration of alpha terpineol with doxorubicin showed strong
synergistic effect (CI 0,1-0,3). Apoptotic assay by flow cytometry Annexin V-Pi
showed alpha terpineol and doxorubicin combination caused 4,5% early apoptotic
cell compared single doxorubicin.
Based on this results, alpha terpineol is a potential compound to developed
as co-chemoterapeutic agent. However, the molecular mechanism need to be
explored further.
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