PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7
Alpha terpineol is a naturally alcoholic-monoterpene compound. This component is a part of essential oil extract in plants, such as Pines (Pinus merkusii). Alpha terpineol is a potential natural product with anticancer activity which is proven can decrease cells growth and induces cells death. Chemo...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| author | , RIA FAJARWATI , Prof. dr. Sofia Mubarika, H., M.Med.Sc, Ph.D |
| author_facet | , RIA FAJARWATI , Prof. dr. Sofia Mubarika, H., M.Med.Sc, Ph.D |
| author_sort | , RIA FAJARWATI |
| collection | UGM |
| description | Alpha terpineol is a naturally alcoholic-monoterpene compound. This
component is a part of essential oil extract in plants, such as Pines (Pinus merkusii).
Alpha terpineol is a potential natural product with anticancer activity which is
proven can decrease cells growth and induces cells death. Chemotherapeutic
combination is a combined of a chemoprevention compound with chemotherapeutic
agent to increase therapeutic effect and reducing toxicity. The purpose of this study
is to determine the effect combination alpha terpineol with doxorubicin in breast
cancer cell line MCF-7
The cytotoxic assay of alpha terpineol and doxorubicin were carried out by
MTT method using alpha terpineol and doxorubicin concentration of 1,5625-50
µg/ml to determine IC50. The cytotoxic assay of their combination were carried out
by MTT method using concentration were 1/2, 1/8 and 1/20 IC50 values. Apoptotic
assay of their best combination concentration were conducted using flow
cytometryAnnexin V-Pi.
Alpha terpineol and doxorubicin showed cytotoxic effect on MCF-7 breast
cancer cell with IC50 30 µg/ml and 2 µg/ml, respectively. Based on CI values, all
combination concentration of alpha terpineol with doxorubicin showed strong
synergistic effect (CI 0,1-0,3). Apoptotic assay by flow cytometry Annexin V-Pi
showed alpha terpineol and doxorubicin combination caused 4,5% early apoptotic
cell compared single doxorubicin.
Based on this results, alpha terpineol is a potential compound to developed
as co-chemoterapeutic agent. However, the molecular mechanism need to be
explored further. |
| first_indexed | 2024-03-13T23:03:38Z |
| format | Thesis |
| id | oai:generic.eprints.org:123464 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:03:38Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1234642016-03-04T08:42:18Z https://repository.ugm.ac.id/123464/ PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7 , RIA FAJARWATI , Prof. dr. Sofia Mubarika, H., M.Med.Sc, Ph.D ETD Alpha terpineol is a naturally alcoholic-monoterpene compound. This component is a part of essential oil extract in plants, such as Pines (Pinus merkusii). Alpha terpineol is a potential natural product with anticancer activity which is proven can decrease cells growth and induces cells death. Chemotherapeutic combination is a combined of a chemoprevention compound with chemotherapeutic agent to increase therapeutic effect and reducing toxicity. The purpose of this study is to determine the effect combination alpha terpineol with doxorubicin in breast cancer cell line MCF-7 The cytotoxic assay of alpha terpineol and doxorubicin were carried out by MTT method using alpha terpineol and doxorubicin concentration of 1,5625-50 µg/ml to determine IC50. The cytotoxic assay of their combination were carried out by MTT method using concentration were 1/2, 1/8 and 1/20 IC50 values. Apoptotic assay of their best combination concentration were conducted using flow cytometryAnnexin V-Pi. Alpha terpineol and doxorubicin showed cytotoxic effect on MCF-7 breast cancer cell with IC50 30 µg/ml and 2 µg/ml, respectively. Based on CI values, all combination concentration of alpha terpineol with doxorubicin showed strong synergistic effect (CI 0,1-0,3). Apoptotic assay by flow cytometry Annexin V-Pi showed alpha terpineol and doxorubicin combination caused 4,5% early apoptotic cell compared single doxorubicin. Based on this results, alpha terpineol is a potential compound to developed as co-chemoterapeutic agent. However, the molecular mechanism need to be explored further. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , RIA FAJARWATI and , Prof. dr. Sofia Mubarika, H., M.Med.Sc, Ph.D (2013) PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63575 |
| spellingShingle | ETD , RIA FAJARWATI , Prof. dr. Sofia Mubarika, H., M.Med.Sc, Ph.D PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7 |
| title | PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7 |
| title_full | PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7 |
| title_fullStr | PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7 |
| title_full_unstemmed | PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7 |
| title_short | PENINGKATAN SITOTOKSISITAS DOXORUBICIN SEBAGAI AGEN KEMOTERAPI OLEH SENYAWA ALFA TERPINEOL PADA SEL MCF-7 |
| title_sort | peningkatan sitotoksisitas doxorubicin sebagai agen kemoterapi oleh senyawa alfa terpineol pada sel mcf 7 |
| topic | ETD |
| work_keys_str_mv | AT riafajarwati peningkatansitotoksisitasdoxorubicinsebagaiagenkemoterapiolehsenyawaalfaterpineolpadaselmcf7 AT profdrsofiamubarikahmmedscphd peningkatansitotoksisitasdoxorubicinsebagaiagenkemoterapiolehsenyawaalfaterpineolpadaselmcf7 |