| Summary: | Infectious coryza (snot) is one of upper respiratory disease in chiken
caused by Avibacterium paragallinarum (Av. paragallinarum) and can be found
in acute to chronic form. Avibacterium paragallinarum has strains with different
antigenicity and up to 3 serotypes currently known, namely A, B and C. This
study was designed to isolate, identify and to determine the serotype of Av.
Paragallinarum isolated from comercial layer with symptoms of snot. Samples
were taken from 12 layers which showing symptoms of snot. Samples from
sinuses infected were cultured on chocolate agar and observed colony
morphology. Gram staining and biochemical tests (catalase, oxidase, H2S, urea,
and fermentations of carbohydrates) were performed on suspected colonies.
Colonies that showed Gram negative and biochemical tests indicated Av.
Paragallinarum, were serotyping by plate agglutination method and followed by
antibiotics sensitivity test. Results of this experiment showed four positive isolates
Av. paragallinarum with 2 isolates are Av. paragallinarum serotype B and two
other isolates are serotype C. Further, antibiotic sensitivity test revealed that Av.
paragallinarum were sensitive to combination of amoxicillin-clavulanic acid
(100%) and ampicillin (100%), gentamicin and penicillin (75%), kanamycin
(25%)
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