AKTIVITAS INHIBISI α-AMILASE DAN α-GLUKOSIDASE OLEH EKSTRAK POLISAKARIDA DAN SENYAWA POLIFENOL DARI Eucheuma denticulatum DAN Sargassum hystrix
Alginate and polyphenol extract of Sargassum histrix and carrageenan and polyphenols extract of Eucheuma denticulatum were tested for their ability to inhibit carbohydrate hydrolysis enzymes (α-amylase and α-glucosidase) by in vitro methods. Extraction of alginate from Eucheuma denticulatum was us...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| Summary: | Alginate and polyphenol extract of Sargassum histrix and carrageenan and
polyphenols extract of Eucheuma denticulatum were tested for their ability to
inhibit carbohydrate hydrolysis enzymes (α-amylase and α-glucosidase) by in
vitro methods. Extraction of alginate from Eucheuma denticulatum was using
0.5% KOH solution. Sodium alginate was extracted from Sargassum histrix by
using 4% Na2CO3 solution. Polyphenols were extracted from both marine algae
by using 50% (v/v). Identification of carrageenan and alginate was determined by
Fourier Transform Infrared (FTIR) spectroscopy. Infrared spectra of
polysaccharide standards (κ- carrageenan standard and sodium alginate standard)
were obtained as comparison. Total polyphenols contents from the extracts were
determined according to the Folin-Ciocalteu method. Carrageenan and
polyphenols extracts from Eucheuma denticulatum showed the ability to inhibit
the activity of α-amylase with an IC50 12.16 and 11.64 mg / mL and no inhibit the
activity of α-glucosidase with an IC50. Alginate and polyphenols extracts from
Sargassum hystrix showed the ability to inhibit the activity of α-amylase with an
IC50 15.75 and 11.26 mg / mL and polyphenols inhibit the activity of α-
glucosidase with an IC50 9.57 mg / mL. Polyphenols extract from Sargassum
hystrix were more effective to inhibit α-amylase and α-glucosidase than extract
alginate from Sargassum hystrix, and carrageenan and polyphenols extract from
Eucheuma denticulatum. |
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