ISOLASI ENZIM SELULASE dari TRICHODERMA B7 MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL
Research has been conducted isolate of the fungus Trichoderma B7cellulase enzyme potentially produce cellulase enzymes mainly endoglukanase, so research must be done to isolate the cellulase enzyme fermentation produc from the fungus Trichoderma B7 were inoculated on media Mendel, carbon source from...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| author | , Ika Fitriana, STP. , Dr. Ir. M. Nur Cahyanto, M.Sc. |
| author_facet | , Ika Fitriana, STP. , Dr. Ir. M. Nur Cahyanto, M.Sc. |
| author_sort | , Ika Fitriana, STP. |
| collection | UGM |
| description | Research has been conducted isolate of the fungus Trichoderma B7cellulase enzyme potentially produce cellulase enzymes mainly endoglukanase, so research must be done to isolate the cellulase enzyme fermentation produc from the fungus Trichoderma B7 were inoculated on media Mendel, carbon source from CMC (Carboxy methyl cellulose sodium salt), fermentation Submerge for 5 days and agitation of 120 rpm at a temperature of 30 � C.
The enzymes from fermentation process isolate using ammonium sulfate at levels of 50% ethanol, 60%, 70%, 80%, pH 4.5 and a temperature of 0 � C. Then do the testing for protein content, enzyme activity, the specific activity of the enzyme cellulase after fermentation and after precipitation. Then calculated value of yields and purify of enzymes.
Results of research the cellulase enzymes after isolation by using saturated ammonium sulfate precipitation optimum activity of CMCase 0,189 mU / ml, protein content 19,797 mg / ml in concentration levels of precipitation 80%. At optimum levels of precipitation 50% The specific activity of CMCase 0,034 mU / mg yield of 89,6%, and Purity 0,74.
Cellulase enzymes after isolated by precipitation ethanol optimum at levels of precipitation 50%, 0,198 CMCase activity mU / ml, CMCase The specific activity of 0,083 mU / mg, 93,83% yield and purity 2.52. Optimum levels of precipitation 70% protein content 4,118 mg / ml. |
| first_indexed | 2024-03-13T23:04:43Z |
| format | Thesis |
| id | oai:generic.eprints.org:123759 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:04:43Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1237592016-03-04T08:39:10Z https://repository.ugm.ac.id/123759/ ISOLASI ENZIM SELULASE dari TRICHODERMA B7 MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL , Ika Fitriana, STP. , Dr. Ir. M. Nur Cahyanto, M.Sc. ETD Research has been conducted isolate of the fungus Trichoderma B7cellulase enzyme potentially produce cellulase enzymes mainly endoglukanase, so research must be done to isolate the cellulase enzyme fermentation produc from the fungus Trichoderma B7 were inoculated on media Mendel, carbon source from CMC (Carboxy methyl cellulose sodium salt), fermentation Submerge for 5 days and agitation of 120 rpm at a temperature of 30 � C. The enzymes from fermentation process isolate using ammonium sulfate at levels of 50% ethanol, 60%, 70%, 80%, pH 4.5 and a temperature of 0 � C. Then do the testing for protein content, enzyme activity, the specific activity of the enzyme cellulase after fermentation and after precipitation. Then calculated value of yields and purify of enzymes. Results of research the cellulase enzymes after isolation by using saturated ammonium sulfate precipitation optimum activity of CMCase 0,189 mU / ml, protein content 19,797 mg / ml in concentration levels of precipitation 80%. At optimum levels of precipitation 50% The specific activity of CMCase 0,034 mU / mg yield of 89,6%, and Purity 0,74. Cellulase enzymes after isolated by precipitation ethanol optimum at levels of precipitation 50%, 0,198 CMCase activity mU / ml, CMCase The specific activity of 0,083 mU / mg, 93,83% yield and purity 2.52. Optimum levels of precipitation 70% protein content 4,118 mg / ml. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Ika Fitriana, STP. and , Dr. Ir. M. Nur Cahyanto, M.Sc. (2013) ISOLASI ENZIM SELULASE dari TRICHODERMA B7 MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63873 |
| spellingShingle | ETD , Ika Fitriana, STP. , Dr. Ir. M. Nur Cahyanto, M.Sc. ISOLASI ENZIM SELULASE dari TRICHODERMA B7 MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL |
| title | ISOLASI ENZIM SELULASE dari TRICHODERMA B7
MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL |
| title_full | ISOLASI ENZIM SELULASE dari TRICHODERMA B7
MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL |
| title_fullStr | ISOLASI ENZIM SELULASE dari TRICHODERMA B7
MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL |
| title_full_unstemmed | ISOLASI ENZIM SELULASE dari TRICHODERMA B7
MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL |
| title_short | ISOLASI ENZIM SELULASE dari TRICHODERMA B7
MENGGUNAKAN AMMONIUM SULFAT dan ETHANOL |
| title_sort | isolasi enzim selulase dari trichoderma b7 menggunakan ammonium sulfat dan ethanol |
| topic | ETD |
| work_keys_str_mv | AT ikafitrianastp isolasienzimselulasedaritrichodermab7menggunakanammoniumsulfatdanethanol AT drirmnurcahyantomsc isolasienzimselulasedaritrichodermab7menggunakanammoniumsulfatdanethanol |