| Summary: | The main etiology of periodontal disease is bacteria which can be found on
plaque. The existence of Porphyromonas gingivalis on subgingival plaque become
the main etiology of periodontitis. Plaque control can be done by using natural
products that contain antibacterial agents. Papaya leaf (Carica papaya L.)
contains flavonoids, tannins, alkaloids, saponins, triterpenoids, and steroids as
antibacterial agents. The aim of this study was to determine the effect of papaya
leaf extract on the growth of Porphyromonas gingivalis bacterial plaque in vitro.
The study was using diffusion well method using 20 samples with 4 TSA petri
dishes that had been swabbed with Porphyromonas gingivalis bacterial
suspension then 5 wells diameter 5 mm was made. Every wells were dropped by 3
treatment groups (papaya leaf extract concentration 2,5%, 5%, 10%), a negative
control group (tween 2,5%), and positive control group (clorhexidine 0,2% mouth
wash). TSA media was incubated at temperature 37ºC during on 24 hours. The
diameter of inhibition zone was showed around the wells as aclear area, then the
diameter was measured by using a sliding caliper to the nearest 0,1 mm as many
as 3 times and the mean was calculated. The data obtained then analyze by using
one way ANOVA and LSD0,05.
The result of one way ANOVA test showed the effect of papaya leaf extract
toward Porphyromonas gingivalis bacterial plaque inhibition zone (p<0,05).
LSD0,05 test showed a significant difference (p<0,05) between negative control
and papaya leaf extract consentration 2,5%, 5%, and 10%, furthermore between
papaya leaf extract consentration 2,5%, 5%, and 10% could not be found the
significant difference (p>0,05). The conclusion of this study was that the papaya
leaf extract could inhibit the growth of Porphyromonas gingivalis bacterial
plaque in vitro.
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