| Summary: | The aim of this research was to determine the effectiveness of oral administrations
of fucoidan in catfish to control MAS (Motile Aeromonas Septicemia). Fucoidan was
extracted from Sargassum sp. by acidic method, then components were analyzed by TLC
stationary phase: silica gel, mobile phase: isopropanol, ethyl acetate and water 7:2:1, and
FT-IR Spectroscopy. This research used Completely Randomized Design: 4 treatments and
3 replications. The treatments were 0 (control), 2, 4, and 6 g of fucoidan/kg of feed which
were given 5 % of the fish biomass in dry base of feed. TLC (Thin Layer Chromatography)
analysis showed that fucoidan isolated contained fucosa comparable to fucose of fucoidan
standard with Rf 0.51. FT-IR spectrum showed that fucoidan from Sargassum sp. have
some functional groups O-H, C=C=O, O-C-O and C-O. Challenge test was performed one
week after the administration of fucoidan orally. Injection of A. hydrophila KP1 was done
by intraperitoneal injection at 0.1 ml/fish with a density of 2.77 x10
7
cells/ml.
Observations of mortality and symptoms were performed for 7 days after infection. The
results showed that administration of fucoidan orally at a dose of 2, 4, and 6 g of
fucoidan/kg feed did not increase the survival rate (P>0.05) and could not delay MTD
(Mean Time to Death) (P>0.05) when the fish was challenged with A. hydrophila at
concentrations under LD50
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