ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN

The objectives of the research were to isolate, to identify Streptococcus sp. and to determine the causative disease of their pathogenicity on tilapia (Oreochromis sp.). The samples were collected from three different areas in Sleman and Klaten Regency. The bacteria were isolated from the samples th...

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Main Authors: , ROSA EIRE REZEKI, , Dr.Ir. Murwantoko, M.Si.
Format: Thesis
Published: [Yogyakarta] : Universitas Gadjah Mada 2013
Subjects:
ETD
_version_ 1826047279022211072
author , ROSA EIRE REZEKI
, Dr.Ir. Murwantoko, M.Si.,
author_facet , ROSA EIRE REZEKI
, Dr.Ir. Murwantoko, M.Si.,
author_sort , ROSA EIRE REZEKI
collection UGM
description The objectives of the research were to isolate, to identify Streptococcus sp. and to determine the causative disease of their pathogenicity on tilapia (Oreochromis sp.). The samples were collected from three different areas in Sleman and Klaten Regency. The bacteria were isolated from the samples then continue by postulate Koch test by intramuscular injection at 0,1 ml/fish and observed for it�s symptoms. The characterization and identification were done from morphology of bacterial colony and cell, physiological and biochemical properties. Pathogenicity test were done by injection at the various concentration as 104,106,108cfu/fish into the tilapia with size range of 5 to 7 cm and determined it�s Lethal Dosage value of 50 (LD50). The 32 isolates have been purified and 25 isolates among them were Streptococcus sp .Twelve isolates confirmed as pathogens which divided into 8 isolates (CK II.1, MK III.2, JT V.1, JT V.2, JT VI, JT VII.2, JT VIII, and JT IX.2.) were identified as S. agalactiae and 4 isolates (CK I.1, MK IV, MK V.2, and JT V.3) were identified as S. iniae. The general symptoms of Streptococcus sp. including the abnormal swimming behavior and exophthalmos. The isolate of S. agalactiae (CK II.1, MK III.2 and JT VI) were virulent with the LD50 4,36.105
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spelling oai:generic.eprints.org:1247032016-03-04T08:42:37Z https://repository.ugm.ac.id/124703/ ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN , ROSA EIRE REZEKI , Dr.Ir. Murwantoko, M.Si., ETD The objectives of the research were to isolate, to identify Streptococcus sp. and to determine the causative disease of their pathogenicity on tilapia (Oreochromis sp.). The samples were collected from three different areas in Sleman and Klaten Regency. The bacteria were isolated from the samples then continue by postulate Koch test by intramuscular injection at 0,1 ml/fish and observed for it�s symptoms. The characterization and identification were done from morphology of bacterial colony and cell, physiological and biochemical properties. Pathogenicity test were done by injection at the various concentration as 104,106,108cfu/fish into the tilapia with size range of 5 to 7 cm and determined it�s Lethal Dosage value of 50 (LD50). The 32 isolates have been purified and 25 isolates among them were Streptococcus sp .Twelve isolates confirmed as pathogens which divided into 8 isolates (CK II.1, MK III.2, JT V.1, JT V.2, JT VI, JT VII.2, JT VIII, and JT IX.2.) were identified as S. agalactiae and 4 isolates (CK I.1, MK IV, MK V.2, and JT V.3) were identified as S. iniae. The general symptoms of Streptococcus sp. including the abnormal swimming behavior and exophthalmos. The isolate of S. agalactiae (CK II.1, MK III.2 and JT VI) were virulent with the LD50 4,36.105 [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , ROSA EIRE REZEKI and , Dr.Ir. Murwantoko, M.Si., (2013) ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=64859
spellingShingle ETD
, ROSA EIRE REZEKI
, Dr.Ir. Murwantoko, M.Si.,
ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN
title ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN
title_full ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN
title_fullStr ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN
title_full_unstemmed ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN
title_short ISOLASI DAN IDENTIFIKASI Streptococcus sp. PENYEBAB PENYAKIT PADA NILA MERAH (Oreochromis sp.) DARI KABUPATEN SLEMAN DAN KLATEN
title_sort isolasi dan identifikasi streptococcus sp penyebab penyakit pada nila merah oreochromis sp dari kabupaten sleman dan klaten
topic ETD
work_keys_str_mv AT rosaeirerezeki isolasidanidentifikasistreptococcussppenyebabpenyakitpadanilamerahoreochromisspdarikabupatenslemandanklaten
AT drirmurwantokomsi isolasidanidentifikasistreptococcussppenyebabpenyakitpadanilamerahoreochromisspdarikabupatenslemandanklaten