| Summary: | Immunostimulant is known as an effective method to anhance the non specific immune system in fish. One of marine natural products that can be utilized as immunostimulan isalginate. The objectives of this study were determine the effect of dietary alginate on the survival rate, relative protection level, the mean time to death and growth of catfish. Alginate was extracted from Sargassum sp. collected from Gunung Kidul Beach, analyzed by thin layer chromatography and FTIR compared to standard alginate. This study used a completely randomized design with 5 treatments namely control treatment (P1), 2 g alginate/kg feed (P2), 4 g alginate/kg feed (P3), 6 g alginate/kg feed (P4), and 8 g alginate/kg feed (P5) in triplicates. Parameters analyzed were, survival rate, relative percent survival, mean time to death, and growth. Data were analyzed using ANOVA and Duncan's Multiple Range Test (DMRT). The results showed that addition of alginate in to the diet significantly increase the survival rate (P<0,05 ) after the challenge with A.hydrophila. The higher dose of alginate in the feed, resulted higher survival rate. The addition of alginate to the diet resulted relative percent survivalin the range 0f 26,09-69,56% at doses of 4, 6 and 8 g alginate/ kg feed. Average mean time to death of catfish challenged with A.hydrophila increased significantly (P<0,05) compared with controls. The addition of alginate to the diet also increased growth significantly (P<0,05 ).The addition of alginate to feed increasedthe non spesific immune system of catfish to A.hydrophila infection.
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