Summary: | Microorganisms are the most infection agent who cause diseases. The
purpose of this study was to identify bioactive compounds, to test the activity and
the potential for water-soluble extract of the yellow stem wood (Arcangelisia
flava L.Merr) as antifungal against Candida albicans and Trichophyton
mentagrophytes in vitro.
The powder of kayu kuning stem refluxed for 2 hours with distilled water.
The filtrate was evaporated and than counted the yield. Further qualitative and
quantitative analysis by TLC using the comparative method berberine HCl.
Antifungal activity assays performed with the agar diffusion method, and test the
potential with microdilution method, then read absorbance at a wavelength of 595
nm. To facilitate the visual observation, it used MTT reagent, then the solution is
still clear streaking in to agar media. Absorbance readings then analyzed by oneway
ANOVA, followed by post hoc Tukey HSD test.
The results provide yield of 5.70% w/w. At quantitative analysis showed
the extract contains alkaloids berberine HCl by 1.55 ± 0.12% w/w. In the activity
test, inhibition zone formed an average of C.albicans and T.mentagrophytes
respectively 16.65±4,52 and 6.55±0,05 mm. The MIC values for both fungi are
10 mg/mL, the MBC values for C.albicans is 40 mg/mL and for
T.mentagrophytes is 50 mg/mL . From the analysis with one-way ANOVA, shows
that there are significant differences between the groups of test solution and the
control group of fungi with p=0.020 for C.albicans and p=0.028 for
T.mentagrophytes (p <0.050). The results of Post Hoc Tukey analysis, showed that
both concentrations between groups and between groups of test solutions and
positive controls did not differ significantly because the value of p> 0.050.
|