IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT
Immobilization of lipase on chitosan powder was performed via cross linking technique. The aim of this research is to determine the optimum conditions for the immobilization process. Chitosan was prepared from crab shell through continous deacetylation, and crosslinked with glutaraldehyde. Immobiliz...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
|
| Subjects: |
| _version_ | 1797034449601298432 |
|---|---|
| author | , FANDHI ADI WARDOYO , Dr. Tri Joko Raharjo, M.Si. |
| author_facet | , FANDHI ADI WARDOYO , Dr. Tri Joko Raharjo, M.Si. |
| author_sort | , FANDHI ADI WARDOYO |
| collection | UGM |
| description | Immobilization of lipase on chitosan powder was performed via cross linking technique. The aim of this research is to determine the optimum conditions for the immobilization process. Chitosan was prepared from crab shell through continous deacetylation, and crosslinked with glutaraldehyde. Immobilization of lipase on chitosan powder was studied under the optimum conditions e.i. pH optimum of lipase solubility and mole ratio of chitosan:glutaraldehyde. Immobilized lipase on chitosan powder was tested for the activity, thermal stability, multiple-use stability and kinetic parameters through the hydrolysis reaction of palm oil.
Continous deacetylation produced chitosans A, B and C with deacetylation degrees of 81.34% , 90.23%, and 92.80%, respectively. The optimum pH for enzyme dissolution was obtained at pH 6. Mole ratio of chitosan:glutaraldehyde for the enzyme immobilization reached optimum condition at a ratio of 2:1. Optimum efficiency of immobilization on chitosans A, B, and C, were 53.01 |
| first_indexed | 2024-03-13T23:11:54Z |
| format | Thesis |
| id | oai:generic.eprints.org:125834 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:11:54Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1258342016-03-04T08:35:12Z https://repository.ugm.ac.id/125834/ IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT , FANDHI ADI WARDOYO , Dr. Tri Joko Raharjo, M.Si. ETD Immobilization of lipase on chitosan powder was performed via cross linking technique. The aim of this research is to determine the optimum conditions for the immobilization process. Chitosan was prepared from crab shell through continous deacetylation, and crosslinked with glutaraldehyde. Immobilization of lipase on chitosan powder was studied under the optimum conditions e.i. pH optimum of lipase solubility and mole ratio of chitosan:glutaraldehyde. Immobilized lipase on chitosan powder was tested for the activity, thermal stability, multiple-use stability and kinetic parameters through the hydrolysis reaction of palm oil. Continous deacetylation produced chitosans A, B and C with deacetylation degrees of 81.34% , 90.23%, and 92.80%, respectively. The optimum pH for enzyme dissolution was obtained at pH 6. Mole ratio of chitosan:glutaraldehyde for the enzyme immobilization reached optimum condition at a ratio of 2:1. Optimum efficiency of immobilization on chitosans A, B, and C, were 53.01 [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , FANDHI ADI WARDOYO and , Dr. Tri Joko Raharjo, M.Si. (2013) IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66015 |
| spellingShingle | ETD , FANDHI ADI WARDOYO , Dr. Tri Joko Raharjo, M.Si. IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT |
| title | IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT |
| title_full | IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT |
| title_fullStr | IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT |
| title_full_unstemmed | IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT |
| title_short | IMOBILISASI LIPASE DENGAN TEKNIK TAUT SILANG PADA KITOSAN SERBUK DAN UJI AKTIVITAS HIDROLISISNYA TERHADAP MINYAK KELAPA SAWIT |
| title_sort | imobilisasi lipase dengan teknik taut silang pada kitosan serbuk dan uji aktivitas hidrolisisnya terhadap minyak kelapa sawit |
| topic | ETD |
| work_keys_str_mv | AT fandhiadiwardoyo imobilisasilipasedengantekniktautsilangpadakitosanserbukdanujiaktivitashidrolisisnyaterhadapminyakkelapasawit AT drtrijokoraharjomsi imobilisasilipasedengantekniktautsilangpadakitosanserbukdanujiaktivitashidrolisisnyaterhadapminyakkelapasawit |