| Summary: | This research aims to identify the compounds of the active fractions from the
brown seaweed Sargassum hystrix extract as -glucosidase enzyme inhibitor. The
study includes sampling, identification of the seaweed, extraction, separation by
column chromatography, identification by Liquid Chromatography-Electrospray
Ionization Mass Spectrometry (LC-ESIMS) and activity test. The weight of
fractions I, II and III are 0.03%, 0.05%, and 0.03%, respectively. Identification by
ESI-MS fractions I and II showed the precent of loliolide. Fraction I also
contained halogenated furanone, whereas fraction III 4,4�-dihydroxybiphenyl
compound. The activity test of each fractions carried out in vitro using
-glucosidase enzyme and the substrate of p-nitrophenyl- -D-glucopiranoside.
The product of reaction was measured its absorbance using the Elisa microplate
reader at 405 nm. At the concentration of 40 μg/mL, fraksi I, II and III inhibits the
-glucosidase enzyme activity by 89.8%, 95.0%, and 96.1%, respectively. IC50
values of fractions I, II and III, were 8.9, 6.3, and 7.6 μg/mL, respectively. While
IC50 value of an antidiabetic drugs of akarbose was only 2.1 mg/mL. Statistical
tests-one way ANOVA with 95% confidence level was carried out to distinguish
between the IC50 values of each fraction and fraction against of acarbose, with the
results differ significantly (P < 0.05).
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