IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE
This research aims to identify the compounds of the active fractions from the brown seaweed Sargassum hystrix extract as -glucosidase enzyme inhibitor. The study includes sampling, identification of the seaweed, extraction, separation by column chromatography, identification by Liquid Chromatography...
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Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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author | , LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. |
author_facet | , LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. |
author_sort | , LIS FITRAMADAN |
collection | UGM |
description | This research aims to identify the compounds of the active fractions from the
brown seaweed Sargassum hystrix extract as -glucosidase enzyme inhibitor. The
study includes sampling, identification of the seaweed, extraction, separation by
column chromatography, identification by Liquid Chromatography-Electrospray
Ionization Mass Spectrometry (LC-ESIMS) and activity test. The weight of
fractions I, II and III are 0.03%, 0.05%, and 0.03%, respectively. Identification by
ESI-MS fractions I and II showed the precent of loliolide. Fraction I also
contained halogenated furanone, whereas fraction III 4,4�-dihydroxybiphenyl
compound. The activity test of each fractions carried out in vitro using
-glucosidase enzyme and the substrate of p-nitrophenyl- -D-glucopiranoside.
The product of reaction was measured its absorbance using the Elisa microplate
reader at 405 nm. At the concentration of 40 μg/mL, fraksi I, II and III inhibits the
-glucosidase enzyme activity by 89.8%, 95.0%, and 96.1%, respectively. IC50
values of fractions I, II and III, were 8.9, 6.3, and 7.6 μg/mL, respectively. While
IC50 value of an antidiabetic drugs of akarbose was only 2.1 mg/mL. Statistical
tests-one way ANOVA with 95% confidence level was carried out to distinguish
between the IC50 values of each fraction and fraction against of acarbose, with the
results differ significantly (P < 0.05). |
first_indexed | 2024-03-13T23:11:59Z |
format | Thesis |
id | oai:generic.eprints.org:125858 |
institution | Universiti Gadjah Mada |
last_indexed | 2024-03-13T23:11:59Z |
publishDate | 2013 |
publisher | [Yogyakarta] : Universitas Gadjah Mada |
record_format | dspace |
spelling | oai:generic.eprints.org:1258582016-03-04T08:39:50Z https://repository.ugm.ac.id/125858/ IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE , LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. ETD This research aims to identify the compounds of the active fractions from the brown seaweed Sargassum hystrix extract as -glucosidase enzyme inhibitor. The study includes sampling, identification of the seaweed, extraction, separation by column chromatography, identification by Liquid Chromatography-Electrospray Ionization Mass Spectrometry (LC-ESIMS) and activity test. The weight of fractions I, II and III are 0.03%, 0.05%, and 0.03%, respectively. Identification by ESI-MS fractions I and II showed the precent of loliolide. Fraction I also contained halogenated furanone, whereas fraction III 4,4�-dihydroxybiphenyl compound. The activity test of each fractions carried out in vitro using -glucosidase enzyme and the substrate of p-nitrophenyl- -D-glucopiranoside. The product of reaction was measured its absorbance using the Elisa microplate reader at 405 nm. At the concentration of 40 μg/mL, fraksi I, II and III inhibits the -glucosidase enzyme activity by 89.8%, 95.0%, and 96.1%, respectively. IC50 values of fractions I, II and III, were 8.9, 6.3, and 7.6 μg/mL, respectively. While IC50 value of an antidiabetic drugs of akarbose was only 2.1 mg/mL. Statistical tests-one way ANOVA with 95% confidence level was carried out to distinguish between the IC50 values of each fraction and fraction against of acarbose, with the results differ significantly (P < 0.05). [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , LIS FITRAMADAN and , Respati Tri Swasono, S.Si., M.Phil., Ph.D. (2013) IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66039 |
spellingShingle | ETD , LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE |
title | IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_full | IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_fullStr | IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_full_unstemmed | IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_short | IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_sort | identifikasi senyawa dalam fraksi aktif ekstrak rumput laut coklat sargassum hystrix sebagai inhibitor enzim glukosidase |
topic | ETD |
work_keys_str_mv | AT lisfitramadan identifikasisenyawadalamfraksiaktifekstrakrumputlautcoklatsargassumhystrixsebagaiinhibitorenzimglukosidase AT respatitriswasonossimphilphd identifikasisenyawadalamfraksiaktifekstrakrumputlautcoklatsargassumhystrixsebagaiinhibitorenzimglukosidase |