EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D SERTA POLA KEPEKAAN ANTIBIOTIK ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA) DAN METHICILLIN SENSITIF S. AUREUS (MSSA)
Nowadays, Staphylococcus aureus, especially MRSA isolates, emerged as a major pathogenic agent of nosocomial infection and sepsis worldwide. Infections caused by these bacteria are often very severe and difficult to treat because of the limited sensitivity of antibiotics and the development of antib...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1797034523090747392 |
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| author | , TITIK NURYASTUTI , dr. Praseno, SpMK(K) |
| author_facet | , TITIK NURYASTUTI , dr. Praseno, SpMK(K) |
| author_sort | , TITIK NURYASTUTI |
| collection | UGM |
| description | Nowadays, Staphylococcus aureus, especially MRSA isolates, emerged as a major
pathogenic agent of nosocomial infection and sepsis worldwide. Infections caused by
these bacteria are often very severe and difficult to treat because of the limited
sensitivity of antibiotics and the development of antibiotic resistance is very fast.
Biofilm formation is an important factor in the pathogenicity of Staphylococcus
infections, since the bacteria can attach to and colonizes in biomaterials devices.
Biofilm is one of the reason of antibiotic therapy failure.
In this study, the ability of MRSA isolate (Methicillin Resistant Staphylococcus
aureus) and MSSA (Methicillin Sensitive Staphylococcus aureus) to form biofilm
were studied by congo red agar and microtiter plate test. PCR was performed to detect
the presence of icaA/D, which responsible for biofilm production. Antibiotic
sensitivity was done against MSSA and MRSA isolates by agar diffusion method.
The results of this study showed that 90 % of MRSA isolates were not able to form
biofilm, whereas all MSSA isolates have ability to form biofilm, although all MRSA
and MSSA isolates harbour both genes icaA/D in the chromosomes. MRSA isolates
were more resistant to many classes of antibiotics than MSSA. Antibiotics still potent
for most of the MRSA isolates were chloramphenicol, erythromycin and
cotrimoxazole. There seems to be differences in the mechanisms of regulation and
biofilm formation in MRSA and MSSA isolates. The presence of SSC mec in MRSA
bacterial chromosome, will repress gene controlling of biofilm formation, so MRSA
prefers to express the antibiotic resistance genes which can be used to adapt in clinical
situation. |
| first_indexed | 2024-03-13T23:13:02Z |
| format | Thesis |
| id | oai:generic.eprints.org:126194 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:13:02Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1261942016-03-04T08:43:53Z https://repository.ugm.ac.id/126194/ EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D SERTA POLA KEPEKAAN ANTIBIOTIK ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA) DAN METHICILLIN SENSITIF S. AUREUS (MSSA) , TITIK NURYASTUTI , dr. Praseno, SpMK(K) ETD Nowadays, Staphylococcus aureus, especially MRSA isolates, emerged as a major pathogenic agent of nosocomial infection and sepsis worldwide. Infections caused by these bacteria are often very severe and difficult to treat because of the limited sensitivity of antibiotics and the development of antibiotic resistance is very fast. Biofilm formation is an important factor in the pathogenicity of Staphylococcus infections, since the bacteria can attach to and colonizes in biomaterials devices. Biofilm is one of the reason of antibiotic therapy failure. In this study, the ability of MRSA isolate (Methicillin Resistant Staphylococcus aureus) and MSSA (Methicillin Sensitive Staphylococcus aureus) to form biofilm were studied by congo red agar and microtiter plate test. PCR was performed to detect the presence of icaA/D, which responsible for biofilm production. Antibiotic sensitivity was done against MSSA and MRSA isolates by agar diffusion method. The results of this study showed that 90 % of MRSA isolates were not able to form biofilm, whereas all MSSA isolates have ability to form biofilm, although all MRSA and MSSA isolates harbour both genes icaA/D in the chromosomes. MRSA isolates were more resistant to many classes of antibiotics than MSSA. Antibiotics still potent for most of the MRSA isolates were chloramphenicol, erythromycin and cotrimoxazole. There seems to be differences in the mechanisms of regulation and biofilm formation in MRSA and MSSA isolates. The presence of SSC mec in MRSA bacterial chromosome, will repress gene controlling of biofilm formation, so MRSA prefers to express the antibiotic resistance genes which can be used to adapt in clinical situation. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , TITIK NURYASTUTI and , dr. Praseno, SpMK(K) (2013) EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D SERTA POLA KEPEKAAN ANTIBIOTIK ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA) DAN METHICILLIN SENSITIF S. AUREUS (MSSA). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66396 |
| spellingShingle | ETD , TITIK NURYASTUTI , dr. Praseno, SpMK(K) EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D SERTA POLA KEPEKAAN ANTIBIOTIK ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA) DAN METHICILLIN SENSITIF S. AUREUS (MSSA) |
| title | EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D
SERTA POLA KEPEKAAN ANTIBIOTIK
ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA)
DAN METHICILLIN SENSITIF S. AUREUS (MSSA) |
| title_full | EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D
SERTA POLA KEPEKAAN ANTIBIOTIK
ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA)
DAN METHICILLIN SENSITIF S. AUREUS (MSSA) |
| title_fullStr | EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D
SERTA POLA KEPEKAAN ANTIBIOTIK
ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA)
DAN METHICILLIN SENSITIF S. AUREUS (MSSA) |
| title_full_unstemmed | EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D
SERTA POLA KEPEKAAN ANTIBIOTIK
ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA)
DAN METHICILLIN SENSITIF S. AUREUS (MSSA) |
| title_short | EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D
SERTA POLA KEPEKAAN ANTIBIOTIK
ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA)
DAN METHICILLIN SENSITIF S. AUREUS (MSSA) |
| title_sort | evaluasi pembentukan biofilm distribusi gen icaa d serta pola kepekaan antibiotik isolat methicillin resisten s aureus mrsa dan methicillin sensitif s aureus mssa |
| topic | ETD |
| work_keys_str_mv | AT titiknuryastuti evaluasipembentukanbiofilmdistribusigenicaadsertapolakepekaanantibiotikisolatmethicillinresistensaureusmrsadanmethicillinsensitifsaureusmssa AT drprasenospmkk evaluasipembentukanbiofilmdistribusigenicaadsertapolakepekaanantibiotikisolatmethicillinresistensaureusmrsadanmethicillinsensitifsaureusmssa |