| Summary: | Candida sp is a normal flora in the human being, which may be found in the skin,
gastrointestinal tract and genitourinary tract. Candida consists of many species. Nowadays,
there are more than 200 species of fungi included within this genus. Among genus Candida,
Candida albicans, is the most predominant in the human body. Candida sp responsible for
many diseases in animals and human being. Human immunodeficiency virus (HIV) infected
patient is common to suffer from oropharyngeal condidiasis (OPC). C. albicans is an
opportunistic fungi that possess virulence factors, eg: phenotypic switching, hyphal formation,
and the growth rate.
This study aims to examine the difference of virulence factors of C. albicans isolated
from HIV patients and healthy individual. There are 20 C. albicans isolated from oral cavity
of HIV patients and 14 C. albicans which were isolated from oral cavity of healthy individual
analyzed in this study. The phenotypic switching was detected by using YPD medium
supplemented with phloxin B, and further confirmed using microscopic examination. Hyphal
formation was measured using germ tube tests. The growth rate was analyzed by serial
spectrophotometer measurements at 0, 6, 24, 48, and 72 hours after inoculation in the CYG
broth medium.
Phenotypic switching (white-opaque transition) were observed in C. albicans which
was isolated from HIV patients but not in healthy individual. There are no significant
difference of hyphal formation and growth rate of C. albicans which was isolated from HIV
infected patients and healthy individual.
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