Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular

Purwoceng (Pimpinella pruatjan Molkenb.) is a medicinal plant that has commercial properties as an aphrodisiac, diuretic and tonic. Great exploitation make P. pruatjan becomes rare plants and endangered species as it has been reported by the Convention on International Trading in Endangered Species...

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Bibliografische gegevens
Hoofdauteurs: , AZIZATUR RAHMAH, , Dr. Rina Sri Kasiamdari.
Formaat: Thesis
Gepubliceerd in: [Yogyakarta] : Universitas Gadjah Mada 2013
Onderwerpen:
ETD
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author , AZIZATUR RAHMAH
, Dr. Rina Sri Kasiamdari.
author_facet , AZIZATUR RAHMAH
, Dr. Rina Sri Kasiamdari.
author_sort , AZIZATUR RAHMAH
collection UGM
description Purwoceng (Pimpinella pruatjan Molkenb.) is a medicinal plant that has commercial properties as an aphrodisiac, diuretic and tonic. Great exploitation make P. pruatjan becomes rare plants and endangered species as it has been reported by the Convention on International Trading in Endangered Species of Wild Flora and Fauna (CITES). The study was conducted to study at the genetic variability of P. pruatjan in Java reviewed based on morphological and molecular characters using Inter Simple Sequence Repeat (ISSR). Morphological and molecular characters of all accessions were taken from four different in places such as the Dieng Plateau (Central Java), Putri Mountain (West Java), Ranupane region (East Java) and Lawu Mountains (Central Java). Morphological data retrieval was done by characterizing root, stems, leaves, fruits and seeds. Molecular data was conducted by ISSR method and DNA isolation was done with Qiagen DNeasy Plant Mini kit, while Polymerase Chain Reaction (PCR) with 7 ISSR primers and electrophoresis. Dendrogram showed the character of the vegetative formed two clusters with a similarity value of 90%. Generative morphological characters formed two clusters with similarity value of 82.1%. Molecular character formed four clusters with a similarity value of 77.5%, with an ISSR markers formed ecotypes based on sampling location.
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spelling oai:generic.eprints.org:1263492016-03-04T08:25:11Z https://repository.ugm.ac.id/126349/ Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular , AZIZATUR RAHMAH , Dr. Rina Sri Kasiamdari. ETD Purwoceng (Pimpinella pruatjan Molkenb.) is a medicinal plant that has commercial properties as an aphrodisiac, diuretic and tonic. Great exploitation make P. pruatjan becomes rare plants and endangered species as it has been reported by the Convention on International Trading in Endangered Species of Wild Flora and Fauna (CITES). The study was conducted to study at the genetic variability of P. pruatjan in Java reviewed based on morphological and molecular characters using Inter Simple Sequence Repeat (ISSR). Morphological and molecular characters of all accessions were taken from four different in places such as the Dieng Plateau (Central Java), Putri Mountain (West Java), Ranupane region (East Java) and Lawu Mountains (Central Java). Morphological data retrieval was done by characterizing root, stems, leaves, fruits and seeds. Molecular data was conducted by ISSR method and DNA isolation was done with Qiagen DNeasy Plant Mini kit, while Polymerase Chain Reaction (PCR) with 7 ISSR primers and electrophoresis. Dendrogram showed the character of the vegetative formed two clusters with a similarity value of 90%. Generative morphological characters formed two clusters with similarity value of 82.1%. Molecular character formed four clusters with a similarity value of 77.5%, with an ISSR markers formed ecotypes based on sampling location. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , AZIZATUR RAHMAH and , Dr. Rina Sri Kasiamdari. (2013) Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66572
spellingShingle ETD
, AZIZATUR RAHMAH
, Dr. Rina Sri Kasiamdari.
Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular
title Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular
title_full Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular
title_fullStr Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular
title_full_unstemmed Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular
title_short Hubungan Kekerabatan Purwoceng (Pimpinella pruatjan Molkenb.) di pulau jawa berdasarkan karakter morfologi dan molekular
title_sort hubungan kekerabatan purwoceng pimpinella pruatjan molkenb di pulau jawa berdasarkan karakter morfologi dan molekular
topic ETD
work_keys_str_mv AT azizaturrahmah hubungankekerabatanpurwocengpimpinellapruatjanmolkenbdipulaujawaberdasarkankaraktermorfologidanmolekular
AT drrinasrikasiamdari hubungankekerabatanpurwocengpimpinellapruatjanmolkenbdipulaujawaberdasarkankaraktermorfologidanmolekular