| Resumo: | There is a problem faced in the development of melon (Cucumis melo L.)
in Yogyakarta, which is the presence of leaf curl disease with a specific sym ptom
of Begomovirus infection. The minor information of kinds and patogenic
characteristics of this causing virus is one of the significant problems to find the
strategy to solve the virus problem. The purpose of this research is to molecularly
identify the leaf curl causing virus in melon in Yogyakarta and to find the genetic
relationship of this virus with other Begomovirus members which cause leaf curl
disease which has been published in Genebank database. This research was
conducted through several phases, which are: curly leaves taking on the field,
virus DNA isolation, Begomivirus identification using universal primer Krusty
Homer (Revill et al, 2003), Begomovirus DNA-A and DNA-B detection using
primer Gemini full BamH1 forward and reverse for full genome DNA-A
(Nishigawa, private communication) and primer BF518 and BR1641 (Reddy et al,
2005) for DNA-B, genome sequencing and genetic relationship analysis of the
sequence with other Begomovirus causing leaf curl. The result of field observation
which was conducted in Sewon found some melon plant with symptom of
Begomovirus infection, in early infection which are yellow patterns on the leaves,
and curl on the leaves' edge, and followed by stunted of the plant. The molecular
identification result using PCR shows that leaf curl causing virus in melon is
Begomovirus, having DNA-A and DNA-B. Genetic relationship analysis of this
virus with other Begomovirus causing leaf curl shows that this virus is closely
related with Pepper yellow leaf curl Indonesia virus (AB267834) based on
nucleotide and amino acid sequencing as coat protein of Begomovirus. The result
of this research is the first report of PepYLCIDV infection with bipartite genome
in melon plant with natural leaf curl symptom on Indonesia.
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