IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.)
There is a problem faced in the development of melon (Cucumis melo L.) in Yogyakarta, which is the presence of leaf curl disease with a specific sym ptom of Begomovirus infection. The minor information of kinds and patogenic characteristics of this causing virus is one of the significa...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1826047613452943360 |
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| author | , Fariha Wilisiani , Prof. Dr. Ir. Susamto Somowiyarjo, M.Sc. |
| author_facet | , Fariha Wilisiani , Prof. Dr. Ir. Susamto Somowiyarjo, M.Sc. |
| author_sort | , Fariha Wilisiani |
| collection | UGM |
| description | There is a problem faced in the development of melon (Cucumis melo L.)
in Yogyakarta, which is the presence of leaf curl disease with a specific sym ptom
of Begomovirus infection. The minor information of kinds and patogenic
characteristics of this causing virus is one of the significant problems to find the
strategy to solve the virus problem. The purpose of this research is to molecularly
identify the leaf curl causing virus in melon in Yogyakarta and to find the genetic
relationship of this virus with other Begomovirus members which cause leaf curl
disease which has been published in Genebank database. This research was
conducted through several phases, which are: curly leaves taking on the field,
virus DNA isolation, Begomivirus identification using universal primer Krusty
Homer (Revill et al, 2003), Begomovirus DNA-A and DNA-B detection using
primer Gemini full BamH1 forward and reverse for full genome DNA-A
(Nishigawa, private communication) and primer BF518 and BR1641 (Reddy et al,
2005) for DNA-B, genome sequencing and genetic relationship analysis of the
sequence with other Begomovirus causing leaf curl. The result of field observation
which was conducted in Sewon found some melon plant with symptom of
Begomovirus infection, in early infection which are yellow patterns on the leaves,
and curl on the leaves' edge, and followed by stunted of the plant. The molecular
identification result using PCR shows that leaf curl causing virus in melon is
Begomovirus, having DNA-A and DNA-B. Genetic relationship analysis of this
virus with other Begomovirus causing leaf curl shows that this virus is closely
related with Pepper yellow leaf curl Indonesia virus (AB267834) based on
nucleotide and amino acid sequencing as coat protein of Begomovirus. The result
of this research is the first report of PepYLCIDV infection with bipartite genome
in melon plant with natural leaf curl symptom on Indonesia. |
| first_indexed | 2024-03-13T23:13:35Z |
| format | Thesis |
| id | oai:generic.eprints.org:126366 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:13:35Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1263662016-03-04T07:56:24Z https://repository.ugm.ac.id/126366/ IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.) , Fariha Wilisiani , Prof. Dr. Ir. Susamto Somowiyarjo, M.Sc. ETD There is a problem faced in the development of melon (Cucumis melo L.) in Yogyakarta, which is the presence of leaf curl disease with a specific sym ptom of Begomovirus infection. The minor information of kinds and patogenic characteristics of this causing virus is one of the significant problems to find the strategy to solve the virus problem. The purpose of this research is to molecularly identify the leaf curl causing virus in melon in Yogyakarta and to find the genetic relationship of this virus with other Begomovirus members which cause leaf curl disease which has been published in Genebank database. This research was conducted through several phases, which are: curly leaves taking on the field, virus DNA isolation, Begomivirus identification using universal primer Krusty Homer (Revill et al, 2003), Begomovirus DNA-A and DNA-B detection using primer Gemini full BamH1 forward and reverse for full genome DNA-A (Nishigawa, private communication) and primer BF518 and BR1641 (Reddy et al, 2005) for DNA-B, genome sequencing and genetic relationship analysis of the sequence with other Begomovirus causing leaf curl. The result of field observation which was conducted in Sewon found some melon plant with symptom of Begomovirus infection, in early infection which are yellow patterns on the leaves, and curl on the leaves' edge, and followed by stunted of the plant. The molecular identification result using PCR shows that leaf curl causing virus in melon is Begomovirus, having DNA-A and DNA-B. Genetic relationship analysis of this virus with other Begomovirus causing leaf curl shows that this virus is closely related with Pepper yellow leaf curl Indonesia virus (AB267834) based on nucleotide and amino acid sequencing as coat protein of Begomovirus. The result of this research is the first report of PepYLCIDV infection with bipartite genome in melon plant with natural leaf curl symptom on Indonesia. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Fariha Wilisiani and , Prof. Dr. Ir. Susamto Somowiyarjo, M.Sc. (2014) IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66591 |
| spellingShingle | ETD , Fariha Wilisiani , Prof. Dr. Ir. Susamto Somowiyarjo, M.Sc. IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.) |
| title | IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.) |
| title_full | IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.) |
| title_fullStr | IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.) |
| title_full_unstemmed | IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.) |
| title_short | IDENTIFIKASI MOLEKULER VIRUS PENYEBAB DAUN KERITING PADA MELON (Cucumis melo L.) |
| title_sort | identifikasi molekuler virus penyebab daun keriting pada melon cucumis melo l |
| topic | ETD |
| work_keys_str_mv | AT farihawilisiani identifikasimolekulerviruspenyebabdaunkeritingpadameloncucumismelol AT profdrirsusamtosomowiyarjomsc identifikasimolekulerviruspenyebabdaunkeritingpadameloncucumismelol |