Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences
This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely...
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| Format: | Article |
| Language: | English |
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SPC Beche-de-mer Information Bulletin
2011
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| Online Access: | http://irep.iium.edu.my/12715/1/2011_BDM31_Jan_2011_Kamarul_SM_2010.pdf |
| _version_ | 1796875998921228288 |
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| author | Kamarudin, Kamarul Rahim |
| author_facet | Kamarudin, Kamarul Rahim |
| author_sort | Kamarudin, Kamarul Rahim |
| collection | IIUM |
| description | This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers
(Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) showed the presence of five main genera of sea cucumbers: Molpadia from order Molpadiida and four genera of order Aspidochirotida namely Holothuria, Stichopus, Bohadschia and Actinopyga. All of the 17 species obtained from Malaysia distributed among the main genera except within Actinopyga. Interestingly, Holothuria excellens was out of Holothuria group causing Holothuria to be paraphyletic. High bootstrap value and consistent clustering made
Molpadia, Stichopus, Bohadschia and Actinopyga monophyletic. The relationship of Actinopyga with the other
genera was un-clarified and Stichopus was sister to Molpadia. The latter finding caused the resolution at order level unclear. The pairwise genetic distance calculated using Kimura 2-parameter model further supported and verified findings from the phylogenetic trees. Further studies with more samples and different mitochondrial DNA genes need to be done to get a better view and verification on the molecular phylogeny of sea cucumbers. |
| first_indexed | 2024-03-05T22:46:17Z |
| format | Article |
| id | oai:generic.eprints.org:12715 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:46:17Z |
| publishDate | 2011 |
| publisher | SPC Beche-de-mer Information Bulletin |
| record_format | dspace |
| spelling | oai:generic.eprints.org:127152012-02-08T01:34:57Z http://irep.iium.edu.my/12715/ Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences Kamarudin, Kamarul Rahim QL Zoology This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) showed the presence of five main genera of sea cucumbers: Molpadia from order Molpadiida and four genera of order Aspidochirotida namely Holothuria, Stichopus, Bohadschia and Actinopyga. All of the 17 species obtained from Malaysia distributed among the main genera except within Actinopyga. Interestingly, Holothuria excellens was out of Holothuria group causing Holothuria to be paraphyletic. High bootstrap value and consistent clustering made Molpadia, Stichopus, Bohadschia and Actinopyga monophyletic. The relationship of Actinopyga with the other genera was un-clarified and Stichopus was sister to Molpadia. The latter finding caused the resolution at order level unclear. The pairwise genetic distance calculated using Kimura 2-parameter model further supported and verified findings from the phylogenetic trees. Further studies with more samples and different mitochondrial DNA genes need to be done to get a better view and verification on the molecular phylogeny of sea cucumbers. SPC Beche-de-mer Information Bulletin 2011 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/12715/1/2011_BDM31_Jan_2011_Kamarul_SM_2010.pdf Kamarudin, Kamarul Rahim (2011) Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences. SPC Beche-de-mer Information Bulletin, 31 (Jan). p. 69. ISSN 1025-4943 http://www.spc.int/DigitalLibrary/Doc/FAME/InfoBull/BDM/31/BDM31.pdf |
| spellingShingle | QL Zoology Kamarudin, Kamarul Rahim Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences |
| title | Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences |
| title_full | Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences |
| title_fullStr | Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences |
| title_full_unstemmed | Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences |
| title_short | Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S Mitochondrial rRNA gene sequences |
| title_sort | phylogeny of sea cucumber echinodermata holothuroidea as inferred from 16s mitochondrial rrna gene sequences |
| topic | QL Zoology |
| url | http://irep.iium.edu.my/12715/1/2011_BDM31_Jan_2011_Kamarul_SM_2010.pdf |
| work_keys_str_mv | AT kamarudinkamarulrahim phylogenyofseacucumberechinodermataholothuroideaasinferredfrom16smitochondrialrrnagenesequences |