| Summary: | The aims of this research was to determine the effect of Aeromonas
hydrophila vaccines against immune system of African catfish (Clarias sp.) after
transporting by measuring value of antibody titer, survival rate (SR), mean time of
death (MTD), and specific growth. This research was conducted in four treatments
and two replications with observed factors were vaccination and transportation. The
vaccine was administered by immersion and intraperitoneal injection. Two controls
were applied as unvaccinated and untransported. Immersion vaccination�s method
(P3) was done by dipping the fish in suspension of vaccine in water at 10
8
cells/ml
for thirty minutes. Injection vaccination (P4) was done by injecting vaccine at dose
of 10
8
cells/fish intraperitoneally. The second vaccination (booster) was conducted
after a week and tranporting performed one week later. Antibody titers were
observed before and after vaccination, booster, and transporting. Observations of
the survival rate, and the mean time of death were done for two weeks. The results
showed that vaccines maintain the antibody titer after transporting (P<0,05), reach
up 2
4
whereas the control treatment (non-vaccinated) were just 2
1
. Vaccination also
increased the survival rate (P<0,05) after transporting until 71.6% compare to
control 36.8%. The most effective methods was intraperitoneally injection with the
antibody titer values reached 2
4
, survival rate reached 71.6%, and the mean time of
death was until 150.15 hours. Vaccination also delayed the mean time of death of
fishes (P<0,05). However, the vaccination was not affected on the fish growth rate.
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