POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT
Marker-assisted selection (MAS) is more accurate technique than conventional selection because only individuals which have locus and gene target are selected. The objective of this research was to determine the distribution of parental polymorphism and position of donor segment on whole parental gen...
| Principais autores: | , |
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| Formato: | Tese |
| Publicado em: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Assuntos: |
| _version_ | 1826047817084305408 |
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| author | , HADIANTI DELIANA R , Dr. Panjisalti Basunanda, S.P.,M.P. |
| author_facet | , HADIANTI DELIANA R , Dr. Panjisalti Basunanda, S.P.,M.P. |
| author_sort | , HADIANTI DELIANA R |
| collection | UGM |
| description | Marker-assisted selection (MAS) is more accurate technique than conventional selection
because only individuals which have locus and gene target are selected. The objective of this
research was to determine the distribution of parental polymorphism and position of donor
segment on whole parental genome and also F1 verification associated with the quantiative trait
loci (QTL) for total spikelet number on chromosome 4 (qTSN4), day to heading on chromosome
8 (qDTH8) and Xa7 on chromosome 6. This research was conducted at Indonesian Center for
Agricultural Biotechnology and Genetic Resources Research and Development by February up
to September 2013. Three rice genotypes �Code, IR64-NILs-qTSN4[YP9] and IR64-NILsqDTH8[
YP1] and also their recurrent parent 'IR 64' were analyzed for polimorphism using 412
microsatellite markers and 1 STS marker, M5. Two 'IR 64' near isogenic lines (IR64-NILs) used
as parental for improving �Code� with higher yield, early maturity and also resistant to bacterial
leaf blight. The results show low percentage of polymorphism on two combination parent due to
three genotypes are used derived from 'IR 64'. Introgressed donor segment in �Code� speculate
that carrying genes related spikelet number per panicle besides qTSN4 so that �Code� has
spikelet number per panicle higher and significantly different than �IR 64� based on Dunnett test
W=5%. F1 verification using microsatellite markers RM17483, RM5556, RM6838 and RM20582
represent F1 'Code' � IR64-NILs-qTSN4[YP9] with number of plant 61-73 and also F1 'Code' �
IR64-NILs-qDTH8[YP1] with number of plant 86-100 can be used as backcross material with
'Code' to create BC1F1. |
| first_indexed | 2024-03-13T23:17:02Z |
| format | Thesis |
| id | oai:generic.eprints.org:127397 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:17:02Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1273972016-03-04T07:56:40Z https://repository.ugm.ac.id/127397/ POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT , HADIANTI DELIANA R , Dr. Panjisalti Basunanda, S.P.,M.P. ETD Marker-assisted selection (MAS) is more accurate technique than conventional selection because only individuals which have locus and gene target are selected. The objective of this research was to determine the distribution of parental polymorphism and position of donor segment on whole parental genome and also F1 verification associated with the quantiative trait loci (QTL) for total spikelet number on chromosome 4 (qTSN4), day to heading on chromosome 8 (qDTH8) and Xa7 on chromosome 6. This research was conducted at Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development by February up to September 2013. Three rice genotypes �Code, IR64-NILs-qTSN4[YP9] and IR64-NILsqDTH8[ YP1] and also their recurrent parent 'IR 64' were analyzed for polimorphism using 412 microsatellite markers and 1 STS marker, M5. Two 'IR 64' near isogenic lines (IR64-NILs) used as parental for improving �Code� with higher yield, early maturity and also resistant to bacterial leaf blight. The results show low percentage of polymorphism on two combination parent due to three genotypes are used derived from 'IR 64'. Introgressed donor segment in �Code� speculate that carrying genes related spikelet number per panicle besides qTSN4 so that �Code� has spikelet number per panicle higher and significantly different than �IR 64� based on Dunnett test W=5%. F1 verification using microsatellite markers RM17483, RM5556, RM6838 and RM20582 represent F1 'Code' � IR64-NILs-qTSN4[YP9] with number of plant 61-73 and also F1 'Code' � IR64-NILs-qDTH8[YP1] with number of plant 86-100 can be used as backcross material with 'Code' to create BC1F1. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , HADIANTI DELIANA R and , Dr. Panjisalti Basunanda, S.P.,M.P. (2014) POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=67648 |
| spellingShingle | ETD , HADIANTI DELIANA R , Dr. Panjisalti Basunanda, S.P.,M.P. POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT |
| title | POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT |
| title_full | POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT |
| title_fullStr | POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT |
| title_full_unstemmed | POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT |
| title_short | POLIMORFISME TETUA DAN VERIFIKASI HASIL PERSILANGAN PADI (Oryza sativa L.) �CODE� DENGAN DUA GALUR ISOGENIK �IR 64� MENGGUNAKAN MIKROSATELIT |
| title_sort | polimorfisme tetua dan verifikasi hasil persilangan padi oryza sativa l a��codea�� dengan dua galur isogenik a��ir 64a�� menggunakan mikrosatelit |
| topic | ETD |
| work_keys_str_mv | AT hadiantidelianar polimorfismetetuadanverifikasihasilpersilanganpadioryzasativalacodeadenganduagalurisogenikair64amenggunakanmikrosatelit AT drpanjisaltibasunandaspmp polimorfismetetuadanverifikasihasilpersilanganpadioryzasativalacodeadenganduagalurisogenikair64amenggunakanmikrosatelit |