| Summary: | Osmotolerant Rhizobacteria designated as Enterococcus flavescens has been subjected
to glucose and acid stress condition to study its physiological response. Cells were
cultured in 2 different media : LB (Luria Bertani) and M63 (minimal medium),
supplemented with glucose and aluminium sulphate to imposedouble stress condition.
Physiological responses of the rhizobacteriaunder environmental stress were observed
by measuring its growthandthemetabolitessynthesised. The results of
thisstudydemonstrated that the higher glucose concentration, the lower thecell density in
M63 medium. On the other hand, in M63 medium, elevated concentration of glucose
resulted in higher cell density, despite the fact that the actual cell density in M63
medium was much lower than in LB medium.GC-MS (Gas Chromatography Mass
Spectrophotometer) analysis conductedon cells cultivated using LB medium without
any treatment (control) and on cells grown under LB medium supplemented with
glucose 5% and Al2(SO4)3 500 ppm resulted in several metabolites detected.Two
metabolite compounds were identified in control medium, i.e.oxirane andphenol,
whilein LB medium supplemented with glucose 5% and Al2(SO4)3 500 ppm, nine
metabolite compounds were identified, i.e.phenol, Butanoic acid
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