TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT)

Osmotolerant Rhizobacteria designated as Enterococcus flavescens has been subjected to glucose and acid stress condition to study its physiological response. Cells were cultured in 2 different media : LB (Luria Bertani) and M63 (minimal medium), supplemented with glucose and aluminium sulphate to im...

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Main Authors: , SUMITRI, , Prof.Ir.Triwibowo Yuwono, Ph.D.
Format: Thesis
Published: [Yogyakarta] : Universitas Gadjah Mada 2014
Subjects:
ETD
_version_ 1826047832302288896
author , SUMITRI
, Prof.Ir.Triwibowo Yuwono, Ph.D.
author_facet , SUMITRI
, Prof.Ir.Triwibowo Yuwono, Ph.D.
author_sort , SUMITRI
collection UGM
description Osmotolerant Rhizobacteria designated as Enterococcus flavescens has been subjected to glucose and acid stress condition to study its physiological response. Cells were cultured in 2 different media : LB (Luria Bertani) and M63 (minimal medium), supplemented with glucose and aluminium sulphate to imposedouble stress condition. Physiological responses of the rhizobacteriaunder environmental stress were observed by measuring its growthandthemetabolitessynthesised. The results of thisstudydemonstrated that the higher glucose concentration, the lower thecell density in M63 medium. On the other hand, in M63 medium, elevated concentration of glucose resulted in higher cell density, despite the fact that the actual cell density in M63 medium was much lower than in LB medium.GC-MS (Gas Chromatography Mass Spectrophotometer) analysis conductedon cells cultivated using LB medium without any treatment (control) and on cells grown under LB medium supplemented with glucose 5% and Al2(SO4)3 500 ppm resulted in several metabolites detected.Two metabolite compounds were identified in control medium, i.e.oxirane andphenol, whilein LB medium supplemented with glucose 5% and Al2(SO4)3 500 ppm, nine metabolite compounds were identified, i.e.phenol, Butanoic acid
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institution Universiti Gadjah Mada
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spelling oai:generic.eprints.org:1274742016-03-04T08:13:36Z https://repository.ugm.ac.id/127474/ TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT) , SUMITRI , Prof.Ir.Triwibowo Yuwono, Ph.D. ETD Osmotolerant Rhizobacteria designated as Enterococcus flavescens has been subjected to glucose and acid stress condition to study its physiological response. Cells were cultured in 2 different media : LB (Luria Bertani) and M63 (minimal medium), supplemented with glucose and aluminium sulphate to imposedouble stress condition. Physiological responses of the rhizobacteriaunder environmental stress were observed by measuring its growthandthemetabolitessynthesised. The results of thisstudydemonstrated that the higher glucose concentration, the lower thecell density in M63 medium. On the other hand, in M63 medium, elevated concentration of glucose resulted in higher cell density, despite the fact that the actual cell density in M63 medium was much lower than in LB medium.GC-MS (Gas Chromatography Mass Spectrophotometer) analysis conductedon cells cultivated using LB medium without any treatment (control) and on cells grown under LB medium supplemented with glucose 5% and Al2(SO4)3 500 ppm resulted in several metabolites detected.Two metabolite compounds were identified in control medium, i.e.oxirane andphenol, whilein LB medium supplemented with glucose 5% and Al2(SO4)3 500 ppm, nine metabolite compounds were identified, i.e.phenol, Butanoic acid [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , SUMITRI and , Prof.Ir.Triwibowo Yuwono, Ph.D. (2014) TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=67729
spellingShingle ETD
, SUMITRI
, Prof.Ir.Triwibowo Yuwono, Ph.D.
TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT)
title TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT)
title_full TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT)
title_fullStr TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT)
title_full_unstemmed TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT)
title_short TANGGAPAN FISIOLOGIS RHIZOBAKTERI OSMOTOLERAN (Enterococcus flavescens) TERHADAP CEKAMAN OSMOTIK (GLUKOSA) DAN KEASAMAN (ALUMINIUM SULFAT)
title_sort tanggapan fisiologis rhizobakteri osmotoleran enterococcus flavescens terhadap cekaman osmotik glukosa dan keasaman aluminium sulfat
topic ETD
work_keys_str_mv AT sumitri tanggapanfisiologisrhizobakteriosmotoleranenterococcusflavescensterhadapcekamanosmotikglukosadankeasamanaluminiumsulfat
AT profirtriwibowoyuwonophd tanggapanfisiologisrhizobakteriosmotoleranenterococcusflavescensterhadapcekamanosmotikglukosadankeasamanaluminiumsulfat