| Summary: | Hatchability test done by
each of as many as 25 eggs of Ae . aegypti
Dengue Haemorrhagic Fever is a disease caused by Dengue virus that transmitted
through the bitting of Ae. aegypti mosquito. Vector control has been used in reducing
the cases. The using of larviside in long time will give ecological damages even
resistance. So, many efforts has been done for exploring the botanical larvisides.
Patikan kebo (E. hirta L.) is an herbaceous plant that contains flavonoids, tannins,
alkaloids, polyphenols, and triterpenoids. The objectives of this study was to
determine the effectivities of methanolic extract of the patikan kebo on the egg
hatchability, mortality, and growth of Ae. aegypti larvae.
immersed in a solution of methanolic
extracts of patikan kebo plants concentration 2.000, 4.000, 6.000, 8.000, 10.000, and
12.000 ppm, as well as tap water and abate solution for control groups in different
container, each with 3 replicates . Egg hatchability test and its outgrowth divided into
two treatments, immersed into extract with and without change of media. The bioassay
for susceptibility of insecticides (WHO, 1981) was used in the test of larvae mortality
at the same concentration of egg hatchability test. The results showed that methanolic
extract of patikan kebo give inhibitory effectivities to the egg hatchability up to 85%
(12.000 ppm), the larva outgrowth to the pupae start 96% (2.000 ppm) up to 100%
(6.000 ppm). Inhibition of the pupae outgrowth to the adult mosquito shows 100% at
concentration 2.000 ppm with change of media test and at concentration 6.000 ppm
without change of media. The value of LC50-24 hours and LC50-48 hours is 5.258,16
ppm and 3.444,41 ppm. Increasing concentration of extract caused the higher level of
mortalities.
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