PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK (Kajian in vivo pada Kelinci galur New Zealand)
Micronucleus is one of early markers of DNA damage due to panoramic radiography radiation exposure which might be related to carcinogenesis mechanism. The DNA damage occured because of oxidative reaction that can be detected using 8-oxo-dG expression. β-Carotene is lipid soluble antioxidant substan...
| Main Authors: | , , , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , Rurie Ratna Shantiningsih , Prof. Dr. drg. Munakhir Mudjosemedi, SU., Sp.RKG(K) , Dr. Med. dr. Indwiani Astuti , Prof. Dr. Suwaldi, M.Sc., Apt |
| author_facet | , Rurie Ratna Shantiningsih , Prof. Dr. drg. Munakhir Mudjosemedi, SU., Sp.RKG(K) , Dr. Med. dr. Indwiani Astuti , Prof. Dr. Suwaldi, M.Sc., Apt |
| author_sort | , Rurie Ratna Shantiningsih |
| collection | UGM |
| description | Micronucleus is one of early markers of DNA damage due to panoramic
radiography radiation exposure which might be related to carcinogenesis
mechanism. The DNA damage occured because of oxidative reaction that can be
detected using 8-oxo-dG expression. β-Carotene is lipid soluble antioxidant
substance which is expected to prevent the oxidative reaction and to improve the
juctional communication which detected by Cx-43. The objectives of this study
were to prepare and to develop topical β-Carotene in order to prevent
micronucleus formation on the gingival mucosa due to panoramic radiography
exposure.
The preparation of β-Carotene gingival mucoadhesive patch were done after
analizing the β-Carotene capability to penetrate gingival mucosa. β-Carotene
gingival mucoadhesive patch had been applied to gingival mucosa on the
experimental rabbits. In Group I, the rabbits weren�t applied with the patch.
Group II were applied before, Group III just in the time and Group IV after
panoramic radiography exposure. Micronucleus samples were taken from the
swabbed of rabbit�s gingival mucosa then stained by using modified Feulgen-
Rossenbeck. The 8-oxo-dG and Cx-43 expression were detected using
immunohistochemistry technique. The results were analyzed using descriptive
quantitative analysis, paired t-test, two way anova and Pearson correlation.
β-Carotene gingival mucoadhesif patch could be formed and the β-Carotene
substances would be transported into rabbit�s gingival mucosa.The increasing
number of micronucleus from Group I had different mean (p<0.05) with Group III
and IV. There were significant influences (p<0.05) of β-Carotene gingival
mucoadhesif patch application on Group II and III. It proved that the application
of β-Carotene gingival mucoadhesive prevented the increasing number of
micronucleus especially when it was applied before and during panoramic
radiograpy exposure. In conclusion, the β-Carotene gingival mucoadhesive patch
can be utilized to prevent the panoramic radiography radiation exposure on
rabbit�s gingival mucosa. |
| first_indexed | 2024-03-13T23:18:10Z |
| format | Thesis |
| id | oai:generic.eprints.org:127731 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:18:10Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1277312016-03-04T08:11:32Z https://repository.ugm.ac.id/127731/ PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK (Kajian in vivo pada Kelinci galur New Zealand) , Rurie Ratna Shantiningsih , Prof. Dr. drg. Munakhir Mudjosemedi, SU., Sp.RKG(K) , Dr. Med. dr. Indwiani Astuti , Prof. Dr. Suwaldi, M.Sc., Apt ETD Micronucleus is one of early markers of DNA damage due to panoramic radiography radiation exposure which might be related to carcinogenesis mechanism. The DNA damage occured because of oxidative reaction that can be detected using 8-oxo-dG expression. β-Carotene is lipid soluble antioxidant substance which is expected to prevent the oxidative reaction and to improve the juctional communication which detected by Cx-43. The objectives of this study were to prepare and to develop topical β-Carotene in order to prevent micronucleus formation on the gingival mucosa due to panoramic radiography exposure. The preparation of β-Carotene gingival mucoadhesive patch were done after analizing the β-Carotene capability to penetrate gingival mucosa. β-Carotene gingival mucoadhesive patch had been applied to gingival mucosa on the experimental rabbits. In Group I, the rabbits weren�t applied with the patch. Group II were applied before, Group III just in the time and Group IV after panoramic radiography exposure. Micronucleus samples were taken from the swabbed of rabbit�s gingival mucosa then stained by using modified Feulgen- Rossenbeck. The 8-oxo-dG and Cx-43 expression were detected using immunohistochemistry technique. The results were analyzed using descriptive quantitative analysis, paired t-test, two way anova and Pearson correlation. β-Carotene gingival mucoadhesif patch could be formed and the β-Carotene substances would be transported into rabbit�s gingival mucosa.The increasing number of micronucleus from Group I had different mean (p<0.05) with Group III and IV. There were significant influences (p<0.05) of β-Carotene gingival mucoadhesif patch application on Group II and III. It proved that the application of β-Carotene gingival mucoadhesive prevented the increasing number of micronucleus especially when it was applied before and during panoramic radiograpy exposure. In conclusion, the β-Carotene gingival mucoadhesive patch can be utilized to prevent the panoramic radiography radiation exposure on rabbit�s gingival mucosa. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Rurie Ratna Shantiningsih and , Prof. Dr. drg. Munakhir Mudjosemedi, SU., Sp.RKG(K) and , Dr. Med. dr. Indwiani Astuti and , Prof. Dr. Suwaldi, M.Sc., Apt (2014) PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK (Kajian in vivo pada Kelinci galur New Zealand). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68003 |
| spellingShingle | ETD , Rurie Ratna Shantiningsih , Prof. Dr. drg. Munakhir Mudjosemedi, SU., Sp.RKG(K) , Dr. Med. dr. Indwiani Astuti , Prof. Dr. Suwaldi, M.Sc., Apt PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK (Kajian in vivo pada Kelinci galur New Zealand) |
| title | PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK
(Kajian in vivo pada Kelinci galur New Zealand) |
| title_full | PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK
(Kajian in vivo pada Kelinci galur New Zealand) |
| title_fullStr | PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK
(Kajian in vivo pada Kelinci galur New Zealand) |
| title_full_unstemmed | PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK
(Kajian in vivo pada Kelinci galur New Zealand) |
| title_short | PATCH GINGIVA MUKOADESIF β-CAROTENE SEBAGAI PENCEGAH EFEK SAMPING PAPARAN RADIASI RADIOGRAFI PANORAMIK
(Kajian in vivo pada Kelinci galur New Zealand) |
| title_sort | patch gingiva mukoadesif i² carotene sebagai pencegah efek samping paparan radiasi radiografi panoramik kajian in vivo pada kelinci galur new zealand |
| topic | ETD |
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