| Summary: | Background: VEGF-C is an important cytokine in the
lymphangiogenesis process in breast carcinoma. VEGF-C
is produced by tumor cells and inflammatory cells,
especially TAMs, and after binding with VEGFR-3 it can
stimulate proliferation, differentiation, migration,
and lymphatic endothelial cell maturation. The
relationship between the number of TAMs with VEGF-C
expression in breast carcinoma is unclear.
Objectives: To study the relationship between the
number of peritumoral TAMs with VEGF-C expression in
breast carcinoma cells.
Methods: Forty one breast carcinoma tissue paraffin
blocks were stained with anti-VEGF monoclonal antibody
and anti-CD68 with Streptavidin-biotin complex
technique with DAB chromogen and Hematoxylin Mayer that
expressed CD68 to counterstain. Number of TAMs was
counted from the number of macrophages. Cut-off value
of TAMs were determined and grouped into high and low.
Expression of VEGF-C were assessed on the percentage of
tumor cells, that its cytoplasm is expressing VEGF-C.
Expression of VEGF-C were grouped into low: negative
and positive �10%, high: positive >10%. Relationship
between the number of TAMs with VEGF-C expression was
analyzed by chi-square test.
Results: TAMs cut-off value is 49. The number of
samples TAMs high: 53.7%, low: 46.3%. The number of
samples with expression of VEGF-C <10%: 12.2%, >10%:
87.8%. There was no significant relationship between
the number of TAMS with VEGF-C expression in breast
carcinoma cells (p = 0.762).
Conclusion: In this study, TAMs have not play a role of
VEGF-C induced lymphangiogenesis in breast carcinoma
cells.
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