HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA
Background: VEGF-C is an important cytokine in the lymphangiogenesis process in breast carcinoma. VEGF-C is produced by tumor cells and inflammatory cells, especially TAMs, and after binding with VEGFR-3 it can stimulate proliferation, differentiation, migration, and lymphatic endothelial cell matur...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , RIZKA IBONITA , dr. Irianiwati, Sp.PA(K). |
| author_facet | , RIZKA IBONITA , dr. Irianiwati, Sp.PA(K). |
| author_sort | , RIZKA IBONITA |
| collection | UGM |
| description | Background: VEGF-C is an important cytokine in the
lymphangiogenesis process in breast carcinoma. VEGF-C
is produced by tumor cells and inflammatory cells,
especially TAMs, and after binding with VEGFR-3 it can
stimulate proliferation, differentiation, migration,
and lymphatic endothelial cell maturation. The
relationship between the number of TAMs with VEGF-C
expression in breast carcinoma is unclear.
Objectives: To study the relationship between the
number of peritumoral TAMs with VEGF-C expression in
breast carcinoma cells.
Methods: Forty one breast carcinoma tissue paraffin
blocks were stained with anti-VEGF monoclonal antibody
and anti-CD68 with Streptavidin-biotin complex
technique with DAB chromogen and Hematoxylin Mayer that
expressed CD68 to counterstain. Number of TAMs was
counted from the number of macrophages. Cut-off value
of TAMs were determined and grouped into high and low.
Expression of VEGF-C were assessed on the percentage of
tumor cells, that its cytoplasm is expressing VEGF-C.
Expression of VEGF-C were grouped into low: negative
and positive �10%, high: positive >10%. Relationship
between the number of TAMs with VEGF-C expression was
analyzed by chi-square test.
Results: TAMs cut-off value is 49. The number of
samples TAMs high: 53.7%, low: 46.3%. The number of
samples with expression of VEGF-C <10%: 12.2%, >10%:
87.8%. There was no significant relationship between
the number of TAMS with VEGF-C expression in breast
carcinoma cells (p = 0.762).
Conclusion: In this study, TAMs have not play a role of
VEGF-C induced lymphangiogenesis in breast carcinoma
cells. |
| first_indexed | 2024-03-13T23:18:16Z |
| format | Thesis |
| id | oai:generic.eprints.org:127741 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:18:16Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1277412016-03-04T08:10:02Z https://repository.ugm.ac.id/127741/ HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA , RIZKA IBONITA , dr. Irianiwati, Sp.PA(K). ETD Background: VEGF-C is an important cytokine in the lymphangiogenesis process in breast carcinoma. VEGF-C is produced by tumor cells and inflammatory cells, especially TAMs, and after binding with VEGFR-3 it can stimulate proliferation, differentiation, migration, and lymphatic endothelial cell maturation. The relationship between the number of TAMs with VEGF-C expression in breast carcinoma is unclear. Objectives: To study the relationship between the number of peritumoral TAMs with VEGF-C expression in breast carcinoma cells. Methods: Forty one breast carcinoma tissue paraffin blocks were stained with anti-VEGF monoclonal antibody and anti-CD68 with Streptavidin-biotin complex technique with DAB chromogen and Hematoxylin Mayer that expressed CD68 to counterstain. Number of TAMs was counted from the number of macrophages. Cut-off value of TAMs were determined and grouped into high and low. Expression of VEGF-C were assessed on the percentage of tumor cells, that its cytoplasm is expressing VEGF-C. Expression of VEGF-C were grouped into low: negative and positive �10%, high: positive >10%. Relationship between the number of TAMs with VEGF-C expression was analyzed by chi-square test. Results: TAMs cut-off value is 49. The number of samples TAMs high: 53.7%, low: 46.3%. The number of samples with expression of VEGF-C <10%: 12.2%, >10%: 87.8%. There was no significant relationship between the number of TAMS with VEGF-C expression in breast carcinoma cells (p = 0.762). Conclusion: In this study, TAMs have not play a role of VEGF-C induced lymphangiogenesis in breast carcinoma cells. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , RIZKA IBONITA and , dr. Irianiwati, Sp.PA(K). (2014) HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68015 |
| spellingShingle | ETD , RIZKA IBONITA , dr. Irianiwati, Sp.PA(K). HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA |
| title | HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA |
| title_full | HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA |
| title_fullStr | HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA |
| title_full_unstemmed | HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA |
| title_short | HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA |
| title_sort | hubungan antara tumor associated macrophages tams dengan ekspresi vascular endothelial growth factor c vegf c pada sel karsinoma payudara |
| topic | ETD |
| work_keys_str_mv | AT rizkaibonita hubunganantaratumorassociatedmacrophagestamsdenganekspresivascularendothelialgrowthfactorcvegfcpadaselkarsinomapayudara AT dririaniwatisppak hubunganantaratumorassociatedmacrophagestamsdenganekspresivascularendothelialgrowthfactorcvegfcpadaselkarsinomapayudara |