| Summary: | Background : Tuberculosis (TB) is a disease caused
by the bacterium Mycobacterium tuberculosis. Gold
standard used in the diagnosis of tuberculosis is a
bacterial culture. Gold standard bacterial culture
performed in solid media Lowenstein-Jensen (LJ). The
excess use of LJ solid medium is low cost but it took a
long time. MGIT (Mycobacterium Growth Indicator Tube)
is a growing medium for bacteria Mycobacterium sp.
including M. tuberculosis. The excess of MGIT culture
medium is fast time in diagnosing but susceptible to
bacterial contamination. In this research the presence
of bacterial contaminants in the medium MGIT resolved
by administration of additional antibiotics in MGIT
namely Gentamicin, Piperacillin, or a mixture.
Objective : To determine the effect of antibiotic
Piperacillin, Gentamicin, or mixtures to the growth of
Bacillus sp. in cultures of Mycobacterium tuberculosis
with MGIT liquid medium.
Methods : subject of research were Mycobacterium
tuberculosis and Bacillus sp. were diluted from 10
8
μL/ml to 10
4
μL/ml. The samples used are divided into
two groups, the control group (control M. tuberculosis
and control of Bacillus sp..) And treatment group
(group Piperacillin, Gentamicin groups, and mixed
groups). Incubation at 37 º C, the turbidity observed
visually and by using MGIT reader every day. Then
planted on blood agar and Gram's staining to confirmed
the presence the growth of Bacillus sp.. Ziehl Neelsen
staining was also performed to confirmed the growth of
of Mycobacterium tuberculosis in MGIT tube. Observe the
presence or not gram-negative bacterium bacillus
(Mycobacterium tuberculosis) at 100x magnification
under a microscope.
Results : The results obtained the
administration of Piperacillin can be used to reduce
the growth of Bacillus sp. in culture medium of MGIT.
However, antibiotics gentamicin and the mixture is not
able to reduce the growth of Bacillus sp. in MGIT
medium.
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