| Summary: | Background Occult Hepatitis B Infection (OBI) is the
presence of HBV DNA in liver or circulation without
detectable HBsAg. It makes blood transfusion safety as
a big issues, despite routine screening for HBsAg.
Mutation in a-determinant regio is one of the molecular
mechanism underlying OBI.
Objectives To investigate the prevalence of OBI related
to mutation of a-determinant regio in healthy blood
donors at UPTD RSUP Dr.Sardjito Yogyakarta.
Methods Serum samples of 150 blood donors negative for
HBsAg negative were tested for confirmatory test and
other markers such as anti-HBs and anti-HBc. Samples
were extracted and amplified with nested-PCR methods
then read using electrophoresis. Samples with positive
HBV DNA then were sequenced. The viral load was
determined by quantitative Real Time-PCR.
Result Out of the 150 samples, 18 (12%) were positive
for HBV DNA, consist of 9 (50%) mutation within adeterminant
regio. Of the 9 samples were positive for
mutation within a-determinant regio, 8 (88.9%) were
anti-HBc (+)/ anti-HBs (-).
Conclusion Prevalence of OBI in healthy blood donors at
RSUP Dr. Sardjito Yogyakarta was 12%, and 50% is due to
mutation within a-determinant region.
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