PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr. SARDJITO
Background Occult Hepatitis B Infection (OBI) is the presence of HBV DNA in liver or circulation without detectable HBsAg. It makes blood transfusion safety as a big issues, despite routine screening for HBsAg. Mutation in a-determinant regio is one of the molecular mechanism underlying OBI. Objecti...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , MEGA SUSANTI , dr. Did ik Setyo Heriyanto, Ph.D, Sp.PA |
| author_facet | , MEGA SUSANTI , dr. Did ik Setyo Heriyanto, Ph.D, Sp.PA |
| author_sort | , MEGA SUSANTI |
| collection | UGM |
| description | Background Occult Hepatitis B Infection (OBI) is the
presence of HBV DNA in liver or circulation without
detectable HBsAg. It makes blood transfusion safety as
a big issues, despite routine screening for HBsAg.
Mutation in a-determinant regio is one of the molecular
mechanism underlying OBI.
Objectives To investigate the prevalence of OBI related
to mutation of a-determinant regio in healthy blood
donors at UPTD RSUP Dr.Sardjito Yogyakarta.
Methods Serum samples of 150 blood donors negative for
HBsAg negative were tested for confirmatory test and
other markers such as anti-HBs and anti-HBc. Samples
were extracted and amplified with nested-PCR methods
then read using electrophoresis. Samples with positive
HBV DNA then were sequenced. The viral load was
determined by quantitative Real Time-PCR.
Result Out of the 150 samples, 18 (12%) were positive
for HBV DNA, consist of 9 (50%) mutation within adeterminant
regio. Of the 9 samples were positive for
mutation within a-determinant regio, 8 (88.9%) were
anti-HBc (+)/ anti-HBs (-).
Conclusion Prevalence of OBI in healthy blood donors at
RSUP Dr. Sardjito Yogyakarta was 12%, and 50% is due to
mutation within a-determinant region. |
| first_indexed | 2024-03-13T23:18:52Z |
| format | Thesis |
| id | oai:generic.eprints.org:127937 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:18:52Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1279372016-03-04T07:59:32Z https://repository.ugm.ac.id/127937/ PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr. SARDJITO , MEGA SUSANTI , dr. Did ik Setyo Heriyanto, Ph.D, Sp.PA ETD Background Occult Hepatitis B Infection (OBI) is the presence of HBV DNA in liver or circulation without detectable HBsAg. It makes blood transfusion safety as a big issues, despite routine screening for HBsAg. Mutation in a-determinant regio is one of the molecular mechanism underlying OBI. Objectives To investigate the prevalence of OBI related to mutation of a-determinant regio in healthy blood donors at UPTD RSUP Dr.Sardjito Yogyakarta. Methods Serum samples of 150 blood donors negative for HBsAg negative were tested for confirmatory test and other markers such as anti-HBs and anti-HBc. Samples were extracted and amplified with nested-PCR methods then read using electrophoresis. Samples with positive HBV DNA then were sequenced. The viral load was determined by quantitative Real Time-PCR. Result Out of the 150 samples, 18 (12%) were positive for HBV DNA, consist of 9 (50%) mutation within adeterminant regio. Of the 9 samples were positive for mutation within a-determinant regio, 8 (88.9%) were anti-HBc (+)/ anti-HBs (-). Conclusion Prevalence of OBI in healthy blood donors at RSUP Dr. Sardjito Yogyakarta was 12%, and 50% is due to mutation within a-determinant region. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , MEGA SUSANTI and , dr. Did ik Setyo Heriyanto, Ph.D, Sp.PA (2014) PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr. SARDJITO. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68254 |
| spellingShingle | ETD , MEGA SUSANTI , dr. Did ik Setyo Heriyanto, Ph.D, Sp.PA PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr. SARDJITO |
| title | PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI
REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI
UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr.
SARDJITO |
| title_full | PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI
REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI
UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr.
SARDJITO |
| title_fullStr | PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI
REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI
UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr.
SARDJITO |
| title_full_unstemmed | PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI
REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI
UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr.
SARDJITO |
| title_short | PREVALENSI HEPATITIS B TERSAMAR TERKAIT MUTASI
REGIO ALPHA DETERMINANT PADA PENDONOR DARAH DI
UNIT PELAYANAN TRANSFUSI DARAH RSUP Dr.
SARDJITO |
| title_sort | prevalensi hepatitis b tersamar terkait mutasi regio alpha determinant pada pendonor darah di unit pelayanan transfusi darah rsup dr sardjito |
| topic | ETD |
| work_keys_str_mv | AT megasusanti prevalensihepatitisbtersamarterkaitmutasiregioalphadeterminantpadapendonordarahdiunitpelayanantransfusidarahrsupdrsardjito AT drdidiksetyoheriyantophdsppa prevalensihepatitisbtersamarterkaitmutasiregioalphadeterminantpadapendonordarahdiunitpelayanantransfusidarahrsupdrsardjito |