| Summary: | Tuberculosis (TB) is a disease caused by the bacterium
Mycobacterium tuberculosis. It is the second killer among
infectious diseases after Human Immunodeficiency Virus (HIV).
Use of MGIT (Mycobacterium Growth Indicator Tube) as a culture
medium in the diagnosis of tuberculosis requires a relatively
shorter time compared to solid medium. However, as a liquid
medium, many bacterial contaminants can grow in this medium,
included Proteus sp. Anticipation for the presence of these
contaminants need to be done with the use of broad-spectrum
antibiotics, such as Piperacillin and Gentamicin.
The purpose of this study was to determine the potential of
Piperacillin, Gentamicin, or a mixture (Piperacillin and
Gentamicin) as an anti-contaminant to Proteus spp. in culturing
Mycobacterium tuberculosis by MGIT liquid medium.
This study used cultures of Mycobacterium tuberculosis and
Proteus sp. (104 μg/ml) are available in the Laboratory of
Microbiology, Faculty of Medicine, Gadjah Mada University. The
cultures were performed in a MGIT medium which is divided into a
control group, they are Mycobacterium tuberculosis control and
Proteus spp. control, as well as the treatment groups, they are
Piperacillin group, Gentamicin group, and mixed group with MIC
value was 2 μg/ml for Piperacillin and 8 μg/ml for Gentamicin.
Further, observe the growth of bacteria.
The results of this study was that there is growing of
Mycobacterium tuberculosis and Proteus spp. in Piperacillin
group indicated by the presence of Mycobacterium tuberculosis
specific turbidity and AFB on ZN�s staining for Mycobacterium
tuberculosis, as well as the growth of colonies on MacConkey
culture and gram-negative bacteria on Gram's staining for
Proteus spp. While in the Gentamicin group and mixture group
there were negative of both bacterial growth.
The conclusion of this study is the Piperacillin and
Gentamicin antibiotic is less potential as an anti-contaminant
to Proteus spp. in the culturing of Mycobacterium tuberculosis
by MGIT liquid medium. Further research needs to be done using
broad-spectrum antibiotics in addition to Piperacillin and
Gentamicin.
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