| Summary: | Background: Antibiotic Resitence Bacteria is an enormous
health problem as infectious disease developed. Sometimes
bacteria resists to more than three antibiotics as they
called Multi-Resistant Bacteria. This problem makes
treatment for infectious disease harder. E.Coli is a
bacteria which grows really fast, about every 20 minutes.
It would create a serious problem if E.Coli which cause
infectious disease is a multi-resistant bacteria. This
problem needs a special strategy in using antibiotics so
it can be solved effectively and efficiently. Antibiotic
periodication using is one strategy that has developed
and proved. But number of research that support this
strategy is still low. Research about the pattern of
spontaneous loss of antibiotic resistance in E.Coli could
help support those theory.
Aim: To know the pattern of spontaneous loss of
antibiotic resistance in E.Coli
Method: This research start with grows multi resistance
E.Coli in liquid media (TSB) for a week. There is no
intervention as E.Coli grows in the (TSB), so E.Coli
grows in free-antibiotics milieu. As a week pass by,
E.Coli was subcultured in McConkey agar and their
resistance to antibiotics was tested with disk diffusion
susceptibility test in Mueller Hinton Agar. E.Coli which
lost their resistance then stocked as their subcultured
in McConkey agar. Then the E.Coli that lost their
antibiotic resistance was extracted from stock and have
their DNA isolated. With electroforesis as a final step,
researcher hopes to find that this resistance was caused
by plasmid R.
Results: E.Coli sensitive to cotrimoxazole in day 13th,
to ceftazidime in day 36th, to cefotaxime in day 71st and
x
to ceftriaxone in day 88th. Before, E.Coli was resistance
to those bacterias. Pattern of spontaneous loss of
antibiotics resistance shows that E.Coli have tendency
to become sensitive to antibiotics in one group
consecutively.
Conclusion: Antibiotic resistance in E. Coli could loss
spontaneously. The loss of antibiotic resistance tend to
have a pattern in the same group of antibiotic. It loss
consecutively.
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