| Summary: | Background : P.aeruginosa is one of many bacteria that can cause
Supppurative Chronic Otitis Media. It can form a biofilm which
is difficult to treat because it can defend itself against
various antibiotic.
Objective : The objective of this research was to do a
susceptibility test to a biofilm formed by P.aeruginosa on
Chronic Suppurative Otitis Media patients against gentamicin in
vitro.
Method and Material : Design of this research is observational
analitic, which is cross sectional. Biofilm susceptibility test
against gentamicin done by microbroth dilution method using 96-
well microplate. Concentration of gentamicin was 128, 64, 32,
16, 8, 4, 2, 1, 0.5, and 0.25 g/ml. After putting antibiotic,
MTT assay is performed. Results of MTT assay is analysed with
MBRC50.
Results : There is no concentration founded to have absorbance
below MBRC50 cut-off point. Gentamicin concentration that come
close to MBRC50 cut-off is at 128 g/ml, which can reduce biofilm
cell quantity of P.aeruginosa to the point of 48.5%.
Conclusion : Pseudomonas aeruginosa biofilm on CSOM patients who
came to THT clinic RSUP dr.Sardjito, given the gentamicin in
vitro founded to have highest MBRC of 48.5% at concentration of
128 g/ml.
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